Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 724057
View all beta-1,4-Glucuronyltransferase 1/B4GAT1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human  beta -1,4-Glucuronyltransferase 1/B4GAT1 Antibody
Asp43-Cys415
Accession # O43505

Specificity

Detects human beta -1,4-Glucuronyltransferase 1/B4GAT1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B3GNT2, rhB3GNT6, or rhB4GalT1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

Detection of Human beta -1,4-Glucuronyltransferase 1/B4GAT1 antibody by Western Blot.

Detection of Human beta -1,4-Glucuronyltransferase 1/B4GAT1 by Western Blot.

Western blot shows lysates of IMR-32 human neuroblastoma cell line and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human beta -1,4-Glucuronyltransferase 1/B4GAT1 Monoclonal Antibody (Catalog # MAB6664) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for beta -1,4-Glucuronyltransferase 1/B4GAT1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: IMR‑32 human neuroblastoma cell line and HEK293T human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: beta-1,4-Glucuronyltransferase 1/B4GAT1

B3GNT1 ( beta -1,3-N-acetylglucosaminyltransferase 1; also iGnT) is a 50 kD member of the b3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be incorporated into either N-linked or O-linked glycans. Human B3GNT1 is a 451 amino acid (aa) type II transmembrane protein. It contains an eight aa N-terminal cytoplasmic segment, an extended 28 aa transmembrane region (aa 9-36), and a 379 aa luminal domain (aa 37-415). Over aa 37-415, human B3GNT1 shares 97% aa identity with mouse B3GNT1.

Long Name

beta-1,4-Glucuronyltransferase 1

Alternate Names

B3GNT1, iGAT, iGNT, MDDGA13

Entrez Gene IDs

11041 (Human); 108902 (Mouse); 293667 (Rat)

Gene Symbol

B4GAT1

UniProt

O43505 (Human)

Additional beta-1,4-Glucuronyltransferase 1/B4GAT1 Products

Product Documents for Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

For research use only

Related Research Areas

Glycobiology-related Enzymes

Citations for Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

Customer Reviews for Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

There are currently no reviews for this product. Be the first to review Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody and earn rewards!

Have you used Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies