Detects human beta ‑NGF in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) beta ‑NGF and recombinant rat (rr) beta ‑NGF is observed and less than 5% cross-reactivity with recombinant human (rh) BDNF, rhNT‑3, and rhNT‑4 is observed. This antibody neutralizes the bioactivity of rh beta -NGF, rm beta -NGF, and rr beta -NGF.
Monoclonal Mouse IgG1 Clone # 25623
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human beta -NGF Ser122-Ala241 Accession # CAA36832
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Human beta ‑NGF Biotinylated Antibody (Catalog # BAF256)
Recombinant Human beta-NGF Protein (Catalog # 256-GF)
Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 5 ng/mL Recombinant Human beta ‑NGF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody.
Recombinant Human beta ‑NGF (Catalog # 256-GF) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta ‑NGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human beta -NGF Monoclonal Antibody (Catalog # MAB256). The ND50 is typically 0.1‑0.4 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.
NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.
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