Human beta -NGF Antibody

(4 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human beta -NGF in direct ELISAs and Western blots. In direct ELISAs, greater than 50% cross-reactivity with recombinant mouse
    beta -NGF and recombinant rat beta -NGF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human beta -NGF
    Ser122-Ala241
    Accession # CAA36832
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human beta -NGF (Catalog # 256-GF)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.02-0.08 µg/mL in the presence of 5 ng/mL Recombinant Human beta ‑NGF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody. Recombinant Human beta ‑NGF (Catalog # 256-GF) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta ‑NGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human beta -NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-256-NA). The ND50 is typically
0.02‑0.08 µg/mL.
Immunohistochemistry
beta ‑NGF in Human Brain. beta ‑NGF was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human beta ‑NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-256-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to axons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

  • Long Name:
    beta Nerve Growth Factor
  • Entrez Gene IDs:
    4803 (Human); 18049 (Mouse); 310738 (Rat)
  • Alternate Names:
    Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane
    Authors: Y Dong, A Kanda, K Noda, W Saito, S Ishida
    Sci Rep, 2017;7(0):44266.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  2. Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Gottingen minipig basal forebrain.
    Authors: Fjord-Larsen L, Kusk P, Tornoe J, Juliusson B, Torp M, Bjarkam CR, Nielsen MS, Handberg A, Sorensen JC, Wahlberg LU
    Mol. Ther., 2010;18(12):2164-72.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Nerve growth factor and artemin are paracrine mediators of pancreatic neuropathy in pancreatic adenocarcinoma.
    Authors: Ceyhan GO, Schafer KH, Kerscher AG, Rauch U, Demir IE, Kadihasanoglu M, Bohm C, Muller MW, Buchler MW, Giese NA, Erkan M, Friess H
    Ann. Surg., 2010;251(5):923-31.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Immunodepletion
  4. Atropine-enhanced, antigen challenge-induced airway hyperreactivity in guinea pigs is mediated by eosinophils and nerve growth factor.
    Authors: Verbout NG, Jacoby DB, Gleich GJ, Fryer AD
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L228-37.
    Species: Guinea Pig
    Sample Type: In Vivo
    Application: In vivo
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

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Isotype Controls
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Normal Goat IgG Control

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Secondary Antibodies
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Average Rating: 4 (Based on 1 review)

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Summary

ApplicationImmunoprecipitation
Sample TestedSerum
SpeciesHuman

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