Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BIM Long (BIM L) Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BIML Long (BIM L)
Ala2-Arg120
Accession # NP_006529

Specificity

Detects human BIML in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BIM Long (BIM L) Antibody

BIMLin Human Spleen by Immunohistochemistry (IHC-P).

BIMLin Human Spleen.

BIMLwas detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human BIMLLong (BIM L) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1325) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and perinuclear area in monocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human BIM Long (BIM L) Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Reviewed Applications

Read 1 review rated 5 using AF1325 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BIM Long (BIM L)

BIML (also known as Bod) is one of several splice variants of BIM, a pro-apoptotic protein belonging to the BH3 domain-only subgroup of Bcl-2 family members. BIML is thought to promote apoptosis by binding and inhibiting the activity of anti-apoptoticBcl-2 family members, thereby inducing the release of cytochrome c from mitochondria. BIML is normally sequestered in an inactive conformation from anti-apoptotic Bcl-2 family members through binding to the microtubule-associated dynein motor complex.Certain apoptotic stimuli release BIML from microtubules to neutralize anti-apoptotic Bcl-2 family members, allowing for the initiation of apoptosis.

References

  1. O'Connor, L. et al. (1998) EMBO J. 17:384.
  2. Puthalakath, H. et al. (1999) Mol. Cell. 3:287.
  3. Miyashita, T. (2001) FEBS Letters 509:135.
  4. Strasser, A. (2000) Ann N Y Acad Sci. 917:541.
  5. Marani, M. et al. (2002) Mol. Cell. Biol. 22:3577.
 

Long Name

Bcl-2 Interacting Mediator of Cell Death

Alternate Names

BCL2L11

Entrez Gene IDs

10018 (Human); 12125 (Mouse)

Gene Symbol

BCL2L11

UniProt

NP_006529

Additional BIM Long (BIM L) Products

Product Documents for Human BIM Long (BIM L) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BIM Long (BIM L) Antibody

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Customer Reviews for Human BIM Long (BIM L) Antibody (1)

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