Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 65529
View all BMP-2/BMP-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BMP-2
Ala284-Arg396
Accession # P12643

Specificity

Detects human BMP-2 and human BMP-4 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows no cross‑reactivity with recombinant human (rh) BMP-5 or rhBMP-6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human BMP‑2/BMP‑4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human BMP‑2 (Catalog # 355-BM)
Recombinant Human BMP‑4 (Catalog # 314-BP)

Reviewed Applications

Read 2 reviews rated 5 using MAB355 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-2/BMP-4

BMP-2 and BMP-4 belong to a family of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily, BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs.

Long Name

Bone Morphogenetic Protein 2/4

Alternate Names

BDA2, BMP-2, BMP2A, BMP-2A, BMP2ABone morphogenetic protein 2A, bone morphogenetic protein 2

Entrez Gene IDs

650 (Human); 12156 (Mouse); 29373 (Rat)

Gene Symbol

BMP2

UniProt

P12643

Additional BMP-2/BMP-4 Products

Product Documents for Human BMP‑2/BMP‑4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human BMP‑2/BMP‑4 Antibody

For research use only

Citations for Human BMP‑2/BMP‑4 Antibody

Customer Reviews for Human BMP‑2/BMP‑4 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Human BMP-2/BMP-4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mesenchymal stem cells
    Species: Human
    Verified Customer | Posted 01/18/2022
    Human BMP‑2/BMP‑4 Antibody MAB355
  • Human BMP-2/BMP-4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Tonsil tissue
    Species: Human
    Verified Customer | Posted 05/22/2018

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