hh Human BMP-3b/GDF-10 Antibody AF1543: R&D Systems

Human BMP-3b/GDF-10 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human BMP‑3b/GDF‑10 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) BMP-3 is observed and less than 2% cross-reactivity with rhBMP-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human BMP‑3b/GDF‑10
    Gln369-Arg478
    Accession # Q5VSQ8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human BMP‑3b/GDF‑10 (Catalog # 1543-BP)
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-3b/GDF-10

BMP-3b, also known as GDF-10, belongs to the transforming growth factor beta (TGF-beta ) superfamily which includes the TGF-beta s, bone morphogenetic proteins (BMPs), growth differentiation factors (GDFs), Activins, Inhibins, Leftys, Nodal, Mullerian inhibitory substance (MIS) and the glial cell line-derived neurotrophic factors (GDNFs) (1). TGF-beta family members are synthesized and secreted as homodimeric or heterodimeric prepropeptides that are cleaved by proprotein convertases such as furin to generate the carboxy-terminal mature dimeric protein that contains the characteristic conserved cysteine residues involved in the formation of the cysteine knot domain. Within pro-BMP-3b, three dibasic cleavage sites have been identified. Mature BMP-3B is a disulfide-linked homodimer of the C-terminal 110 amino acids. Among TGF-beta  family members, BMP-3b is most closely related to BMP-3, sharing 83% and 30% amino acid sequence identity in their mature and pro regions, respectively (2, 3). BMP-3b is highly conserved across animal species, the aa sequence of mature human BMP-3b is 98% identical with that of the mouse or rat proteins (2, 3). BMP-3b is expressed in developing skeletal structures of the craniofacial region and the vertebral column, as well as in the adult trachea, aorta, and most abundantly in the cerebellum and uterus (2‑4). Interestingly, in the knock-out mice, no obvious abnormalities have been found in these tissues (4). The biological function of BMP-3b is yet unknown, however studies have implicated it in the differentiation of osteoblasts, augmenting BMP-2 activity (5, 6).

  • References:
    1. Miyazono, K. et al. (2001) J. Cell Physiol. 187:265.
    2. Cunningham, N.S. et al. (1995) Growth Factors 12:99.
    3. Takao, M. et al. (1996) Biochem. Biophys. Res. Comm. 219:656.
    4. Zhao, R. et al. (1999) Dev. Biol. 212:68.
    5. Hino, J. et al. (1999) Biochem.Biophys. Res. Comm. 256:419.
    6. Kaihara, S. et al. (2003) Life Sci. 72:1683.
  • Long Name:
    Bone Morphogenetic Protein 3b
  • Entrez Gene IDs:
    2662 (Human)
  • Alternate Names:
    BIP; BMP3b; BMP-3b; bone morphogenetic protein 3B; Bone-inducing protein; GDF10; GDF-10; growth differentiation factor 10; Growth/differentiation factor 10
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Bone morphogenetic protein-3b (BMP-3b) is expressed in adipocytes and inhibits adipogenesis as a unique complex.
    Authors: Hino J, Miyazawa T, Miyazato M, Kangawa K
    Int J Obes (Lond), 2012;36(5):725-34.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
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