Human BMP‑7 Biotinylated Antibody (Catalog # BAM354)
Recombinant Human BMP-7 Protein (Catalog # 354-BP)
Measured by its ability to neutralize BMP‑7-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Erlacher, L. et al. (1998) J. Bone Miner. Res. 13:383. The Neutralization Dose (ND50) is typically 4-12 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑7 and 50 µg/mL L-ascorbic acid.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Alkaline Phosphatase Production Induced by BMP‑7 and Neutralization by Human BMP‑7 Antibody. Recombinant Human BMP‑7 (Catalog # 354-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542). The ND50 is typically 4-12 µg/mL in the presence of L-ascorbic acid (50 µg/mL).
BMP‑7 in Human Prostate Cancer Tissue. BMP‑7 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
BMP‑7 in Human Prostate. BMP‑7 was detected in immersion fixed paraffin-embedded sections of human prostate using Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BMP-7, or osteogenic protein 1 (OP-1), is one of at least 15
structurally and functionally related BMPs, which are members of the
transforming growth factor beta (TGF-beta ) superfamily. BMPs regulate
cartilage and bone formation, embryogenesis and morphogenesis of
various tissues and organs, and growth, differentiation, chemotaxis and
apoptosis of various cell types. Biologically active BMP-7 is a
disulfide-linked homodimer. Cellular responses to BMP-7 have been shown
to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors.
Bone Morphogenetic Protein 7
Entrez Gene IDs:
655 (Human); 12162 (Mouse); 85272 (Rat)
BMP7; BMP-7; bone morphogenetic protein 7; Eptotermin alfa; OP-1; OP-1OP1; Osteogenic protein 1
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