Human Brk Antibody

Catalog # Availability Size / Price Qty
Brk in Human Lymphoma.
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Product Details
Supplemental Products

Human Brk Antibody Summary

Species Reactivity
Detects human Brk in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human  (rh) YES, rhFyn, and rhSRC is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human Brk
Accession # Q13882
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Brk
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Brk antibody in Human Lymphoma by Immunohistochemistry (IHC-P). View Larger

Brk in Human Lymphoma. Brk was detected in immersion fixed paraffin-embedded sections of human lymphoma using 15 µg/mL Goat Anti-Human Brk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3966) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Brk

BRK (breast tumor kinase; also known as PTK6) is a 50 kDa, intracellular non-receptor tyrosine kinase. It was first identified in human breast tumor cell lines, is frequently over‑expressed in primary breast tumors, and is absent from normal breast tissue. Other sites of expression include colon, small intestine and prostate. Human BRK is 451 amino acids (aa) in length. It contains one SH3 domain (aa 12‑69), one SH2 domain (aa 77‑170) and a Tyr-kinase catalytic domain (aa 183‑444). There are two potential isoforms. The first has a 66 aa extension on the N-terminus, while the second shows the same extension plus a four aa insert after Arg 277. Over aa 1‑70, human BRK is 79% aa identical to mouse Brk.

Long Name
Breast Tumor Kinase
Entrez Gene IDs
5753 (Human); 20459 (Mouse); 366275 (Rat)
Alternate Names
Breast tumor kinase; Brk; BRKprotein-tyrosine kinase 6; EC 2.7.10; EC; FLJ42088; protein-tyrosine kinase BRK; PTK6 protein tyrosine kinase 6; PTK6; Sik; Tksk; Tyrosine-protein kinase BRK

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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