Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1047350
View all BTNL8 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived human BTNL8 protein
Gln18-Lys238
Accession # Q6UX41-1

Specificity

Detects human BTLN8 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human BTNL8 Antibody

BTNL8 in Human Colon.

BTNL8 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB93591) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human BTNL8 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Reviewed Applications

Read 1 review rated 5 using MAB93591 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTNL8

Butyrophilin-like 8 (BTNL8) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL8 is a 500 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain (2). The ECD of human BTNL8 shares 88% sequence identity with the ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues (3). The complete immunological function of BTNL molecules is only beginning to emerge. BTNL8 has been shown to be important in initiation of primary immune responses, suggesting a role in priming of naïve T lymphocytes (3). Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and colon cancer (4). BTNL8 are expressed in colon, lung, testis and neutrophils, and its expression is significantly decreased in ulcerative colitis, colonic tumors as compared to unaffected tissue (4). Soluble BTNL8-Fc fusion protein binds to resting, but not activated T cells. In vitro, BTNL8 co-stimulates T cell proliferation and cytokine production. In vivo injections of BTNL8-Fc significantly increases production of
Ag-specific IgG during the primary but not the secondary immune response (3).

References

  1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
  2. Arnett, H.A. et al. (2009) Cytokine 46:370.
  3. Chapoval, A.I. et al. (2013) Mol Immunol. 56:819.
  4. Lebrero-Fernández C. et al. (2016) Immun Inflamm Dis. 4:191.

Long Name

Butyrophilin-like 8

Alternate Names

BTN9.2

Entrez Gene IDs

79908 (Human); 406160 (Rat)

Gene Symbol

BTNL8

UniProt

Q6UX41-1

Additional BTNL8 Products

Product Documents for Human BTNL8 Antibody

Certificate of Analysis

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Product Specific Notices for Human BTNL8 Antibody

For research use only

Customer Reviews for Human BTNL8 Antibody (1)

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  • Human BTNL8 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Squamous cell carcinoma
    Species: Human
    Verified Customer | Posted 10/07/2022
    Human BTNL8 Antibody MAB93591

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