Human Cadherin‑13 Antibody

Detection of Human Cadherin‑13 by Simple Western^TM.

Simple Western lane view shows lysates of human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Cadherin-13 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human Cadherin-13 by Western Blot

Differential expression of cadherin 13 and cadherin 4 proteins in 46BR.1G1 and 7A3 cells.(A) Cell lysates from 46BR.1G1 and 7A3 cells were analyzed by Western blotting with anti-cadherin 13, anti-cadherin 4, and anti-alpha -tubulin antibodies. (B) Quantification of the assay was performed by densitometric analysis with NIH ImageJ 1.43 program. Bars show mean ± SEM of three independent experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0130561), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human Cadherin-13 by Western Blot

Differential expression of cadherin 13 and cadherin 4 proteins in 46BR.1G1 and 31W cells.Cell lysates from 46BR.1G1 and 31W cells were analyzed by Western blotting with antibodies against the indicated proteins. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0130561), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Cadherin-13 by Western Blot

HIF-1 activation-induced T-cadherin upregulation increased adiponectin accumulation and EV production. (A) Western blot analysis of total cell lysates. UV-F2 cells cultured in DMEM containing 5% serum from adiponectin knockout mice were treated with or without high molecular weight adiponectin (HMW-APN) (10 μg/mL) and roxadustat (Roxa) (50 μM) for 48 h (n = 3 for each group). (B) Western blot analysis of EVs isolated from cell culture medium by differential ultracentrifugation. UV-F2 cells cultured in FBS-free Advanced DMEM were treated with or without HMW-APN (20 μg/mL) and roxadustat (50 μM) for 48 h (n = 3 for each group). Alix, TSG101, and syntenin were evaluated as EV markers. (C) Western blot analysis of total cell lysates. UV-F2 cells transfected control (Cont) or T-cadherin (T-cad) siRNA were cultured in DMEM containing 5% serum from adiponectin knockout mice with or without HMW-APN (20 μg/mL) and roxadustat (50 μM) for 48 h (n = 3 for each group). (D) Western blot analysis of EVs isolated from cell culture medium. UV-F2 cells transfected Cont or T-cad siRNA were cultured in FBS-free Advanced DMEM with or without HMW-APN (20 μg/mL) and roxadustat (50 μM) for 48 h (n = 3). Data are means ± SEMs. *p < 0.05, **p < 0.01, and ***p < 0.001 (Tukey–Kramer test). Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-024-51935-6), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Cadherin-13 by Western Blot

HIF-1 activation upregulated transcription of the T-cadherin mRNA and increased T-cadherin protein in UV-F2 endothelial cells. (A) Quantitative PCR analysis of murine endothelial UV-F2 cells treated with roxadustat (Roxa) for 24 h, daprodustat (Dapro) for 48 h, or deferoxamine (DFO) for 24 h at the indicated concentrations (n = 3 for each group). Data are means ± SEMs. *p < 0.05, **p < 0.01, and ***p < 0.001 versus control (Dunnett's test). (B) Quantitative PCR analysis of UV-F2 cells treated with or without roxadustat (50 μM) and 1 μM of 2-methoxyestradiol (2-ME), an inhibitor of HIF-1, for 24 h (n = 3 for each group). Data are means ± SEMs. *p < 0.05, **p < 0.01, and ***p < 0.001 (Tukey–Kramer test). (C) The constructs used for reporter assay were summarized (left). Luciferase reporter assay using the promoter region of the mouse T-cadherin gene (mCdh13) in HEK293 cells. Luciferase activities 24 h after treatment with or without roxadustat (50 μM) were quantified by calculating Firefly luciferase activity against an internal standard, Renilla luciferase activity (n = 3 for each group). Data are means ± SEMs. *p < 0.05 (unpaired t test). (D) mRNA stability assay using actinomycin D. UV-F2 cells were incubated with or without roxadustat (50 μM) and 5 μg/mL of actinomycin D for the time indicated (n = 3 for each group). (E) and (F) Western blot analysis of total cell lysates. UV-F2 cells were treated with roxadustat (E) or daprodustat (F) for 48 h at the indicated concentrations (n = 3 for each group). Data are means ± SEMs. *p < 0.05, **p < 0.01, and ***p < 0.001 versus control (Dunnett's test or unpaired t test). Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-024-51935-6), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Cadherin‑13 in NCI-H460 cells by Flow Cytometry

NCI-H460 cells were stained with Goat Anti-Human Cadherin‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.