Cannabinoid R1 (CB1R) is a 60 kDa 7TM protein that belongs to the family of G protein-coupled receptors, class A. CB1R is expressed in the central nervous system and upper GI tract, in contrast to CB2R which is expressed by hematopoietic cells. CB1R mediates the behavioral and gut motility effects of cannabinoids. Human CB1R exists in three alternately spliced forms which are distinguished by N-terminal substitutions or deletions. Human CB1R shares 97% amino acid sequence identity with mouse and rat CB1R.
Human Cannabinoid R1/CB1/CNR1 Antibody
R&D Systems | Catalog # MAB3834
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 368302
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Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with human Cannabinoid R1/CB1/CNR1
Met1-Leu472
Accession # NP_057167
Met1-Leu472
Accession # NP_057167
Specificity
Stains Cannabinoid R1/CB1/CNR1-transfected cells but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Cannabinoid R1/CB1/CNR1 Antibody
Detection of Cannabinoid R1/CB1/CNR1 in NS0 Mouse Cell Line Transfected with Human Cannabinoid R1/CB1/CNR1 and eGFP by Flow Cytometry.
NS0 mouse myeloma cell line transfected with human Cannabinoid R1/CB1/CNR1 and eGFP was stained with and either (A) Mouse Anti-Human Cannabinoid R1/CB1/CNR1 Monoclonal Antibody (Catalog # MAB3834) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).Applications for Human Cannabinoid R1/CB1/CNR1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: NS0 mouse myeloma cell line transfected with human Cannabinoid R1/CB1/CNR1 and eGFP
Sample: NS0 mouse myeloma cell line transfected with human Cannabinoid R1/CB1/CNR1 and eGFP
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Cannabinoid R1/CB1/CNR1
Long Name
Cannabinoid Receptor 1
Alternate Names
CANN6, CannabinoidR1, CB-R, CB1, CB1A, CB1K5, CB1R, CNR1, SKR6R
Gene Symbol
CNR1
UniProt
Additional Cannabinoid R1/CB1/CNR1 Products
Product Documents for Human Cannabinoid R1/CB1/CNR1 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Cannabinoid R1/CB1/CNR1 Antibody
For research use only
Related Research Areas
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