Human Cannabinoid R1/CB1/CNR1 Antibody

Catalog # Availability Size / Price Qty
MAB3834
MAB3834-SP
Human Cannabinoid R1/CB1/CNR1 Antibody in Data
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Human Cannabinoid R1/CB1/CNR1 Antibody Summary

Species Reactivity
Human
Specificity
Stains Cannabinoid R1/CB1/CNR1-transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 368302
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human Cannabinoid R1/CB1/CNR1
Met1-Leu472
Accession # NP_057167
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Cannabinoid R1/CB1/CNR1 in NS0 Mouse Cell Line Transfected with Human Cannabinoid R1/CB1/CNR1 and eGFP by Flow Cytometry. View Larger

Detection of Cannabinoid R1/CB1/CNR1 in NS0 Mouse Cell Line Transfected with Human Cannabinoid R1/CB1/CNR1 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Cannabinoid R1/CB1/CNR1 and eGFP was stained with and either (A) Mouse Anti-Human Cannabinoid R1/CB1/CNR1 Monoclonal Antibody (Catalog # MAB3834) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cannabinoid R1/CB1/CNR1

Cannabinoid R1 (CB1R) is a 60 kDa 7TM protein that belongs to the family of G protein-coupled receptors, class A. CB1R is expressed in the central nervous system and upper GI tract, in contrast to CB2R which is expressed by hematopoietic cells. CB1R mediates the behavioral and gut motility effects of cannabinoids. Human CB1R exists in three alternately spliced forms which are distinguished by N-terminal substitutions or deletions. Human CB1R shares 97% amino acid sequence identity with mouse and rat CB1R.

Long Name
Cannabinoid Receptor 1
Entrez Gene IDs
1268 (Human); 12801 (Mouse); 25248 (Rat)
Alternate Names
CANN6; CANN6CB1R; Cannabinoid R1; cannabinoid receptor 1 (brain); CannabinoidR1; CB1; CB1A; CB1K5; CB1R; CB-R; CB-RCB1cannabinoid receptor 1; central cannabinoid receptor; CNR; CNR1; SKR6R

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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