Human Cannabinoid R2/CB2/CNR2 Antibody

MAB36551R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB36551R-100
MAB36551R-SP

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Detection of Cannabinoid R2/CB2/CNR2 in HEK293 Human Cell Line Transfected with Human Cannabinoid R2/CB2/CNR2 and eGFP by Flow Cytometry.

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Human Cannabinoid R2/CB2/CNR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cannabinoid R2/CB2/CNR2 in HEK293 human cell line transfected with human Cannabinoid R2/CB2/CNR2 and not Cannabinoid R1/CB1/CNR1 transfectants in flow cytometry.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 352110R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived transfected with human Cannabinoid R2/CB2/CNR2
Met1-Cys360
Accession # NP_001832

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

Detection of Cannabinoid R2/CB2/CNR2 antibody in HEK293 Human Cell Line Transfected with Human Cannabinoid R2/CB2/CNR2 and eGFP antibody by Flow Cytometry.

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Detection of Cannabinoid R2/CB2/CNR2 in HEK293 Human Cell Line Transfected with Human Cannabinoid R2/CB2/CNR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Cannabinoid R2/CB2/CNR2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Cannabinoid R2/CB2/CNR2 Monoclonal Antibody (Catalog # MAB36551R) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cannabinoid R2/CB2/CNR2

Cannabinoid R2 (Cannabinoid Receptor 2), also called CB2 or CNR2, is a 7TM protein that belongs to the family of G protein-coupled receptors, class A. Cannabinoid R2 is expressed in the periphery, notably by hematopoietic cells, in contrast to Cannabinoid R1 which is expressed predominantly in the central nervous system and gut. Cannabinoid R2 on lymphocytes and macrophages binds cannabinoids and modulates cytokine secretion. With the exception of their divergent C‑terminal tails, human Cannabinoid R2 shares 82% amino acid sequence identity with mouse and rat Cannabinoid R2.

Long Name

Cannabinoid Receptor 2

Entrez Gene IDs

1269 (Human); 12802 (Mouse); 57302 (Rat)

Alternate Names

Cannabinoid R2; cannabinoid receptor 2 (macrophage); CannabinoidR2; CB-2; CB2cannabinoid receptor 2; CB2R; CNR2; CX5; CX5testis-dominant CNR2 isoform CB2; hCB2