Detection of Human Carbonic Anhydrase III by Western Blot.
Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Carbonic Anhydrase III Monoclonal Antibody (Catalog # MAB2185) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Carbonic Anhydrase III at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase III/CA3
Carbonic Anhydrase is a group of metalloproteases that catalyze the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). CA3 is an approximately 29 kDa cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development
but increases rapidly during the last trimester to reach 50‑60% of adult
levels at birth (2). It is present at high levels in skeletal muscle and much lower levels in other tissues, such as cardiac and smooth muscle. The amino acid sequence of human CA3 is 99%, 90% and 92% identical to that of chimpanzee, dog and mouse/rat.
Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.
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