Human Carbonic Anhydrase VI Antibody

Catalog #: MAB29391 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB29391
MAB29391-SP

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Detection of Carbonic Anhydrase VI in OVCAR‑3 Human Cell Line by Flow Cytometry.

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Human Carbonic Anhydrase VI Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbonic Anhydrase VI in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Carbonic Anhydrase 1, 2, 3, 4, 5A, 5B, 7, 8, or 9 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 401809

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase VI
Gln18-Asn308
Accession # EAW71606

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

OVCAR‑3 human ovarian carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Carbonic Anhydrase VI antibody in OVCAR-3 Human Cell Line antibody by Flow Cytometry.

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Detection of Carbonic Anhydrase VI in OVCAR‑3 Human Cell Line by Flow Cytometry. OVCAR-3 human ovarian carcinoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase VI Monoclonal Antibody (Catalog # MAB29391, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intra-cellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase VI

Carbonic Anhydrase catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc‑metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5).

Entrez Gene IDs

765 (Human); 298657 (Rat)

Alternate Names

CA6; Carbonate dehydratase VI; carbonic anhydrase 6; Carbonic Anhydrase VI; carbonic anhydrase VIGUSTIN; CA-VI; EC 4.2.1.1; Gustin; MGC21256; Salivary carbonic anhydrase; Secreted carbonic anhydrase