Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc‑metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5).
Human Carbonic Anhydrase VI Antibody
R&D Systems | Catalog # MAB29391
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Gln18-Asn308
Accession # EAW71606
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Carbonic Anhydrase VI Antibody
Detection of Carbonic Anhydrase VI in OVCAR‑3 Human Cell Line by Flow Cytometry.
OVCAR-3 human ovarian carcinoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase VI Monoclonal Antibody (Catalog # MAB29391, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intra-cellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human Carbonic Anhydrase VI Antibody
CyTOF-ready
Intracellular Staining by Flow Cytometry
Sample: OVCAR‑3 human ovarian carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Carbonic Anhydrase VI
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Murakami, H. and Sly, W. S. (1987) J. Biol. Chem. 262:1382.
- Thatcher, B. J. et al. (1998) Biochem. Biophys. Res. Commun. 250:635.
- Hankin, R. I. et al. (1999) Am. J. Med. Sci. 318:380.
- Karhumaa, P. et al. (2001) Proc. Natl. Acad. Sci. USA. 98:11604.
Alternate Names
Gene Symbol
UniProt
Additional Carbonic Anhydrase VI Products
Product Documents for Human Carbonic Anhydrase VI Antibody
Certificate of Analysis
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Product Specific Notices for Human Carbonic Anhydrase VI Antibody
For research use only
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