Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 384014
View all Carboxypeptidase A2/CPA2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A2/CPA2
Tyr17-His417
Accession # P48052

Specificity

Detects human Carboxypeptidase A2/CPA2 in direct ELISAs and Western blots. In Western blots, recognizes the pro form but not the activated enzyme.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Carboxypeptidase A2/CPA2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A2/
CPA2 (Catalog # 2896-ZN), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Carboxypeptidase A2 (Catalog # 2896-ZN)

Neutralization

Measured by its ability to neutralize Recombinant Human Carboxypeptidase A2/CPA2 (0.1 µg/mL, Catalog # 2896‑ZN) cleavage of the fluorogenic peptide substrate N-acetyl-Phe-Thiaphe-OH (0.1 mM). The Neutralization Dose (ND50) is typically 1.5 µg/mL.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carboxypeptidase A2/CPA2

Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (residues 1 to 16), a pro region (residues 17 to 112), and a mature chain (residues 113 to 417).

References

  1. Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 821, Academic Press, San Diego.

Alternate Names

CPA2

Entrez Gene IDs

1358 (Human)

Gene Symbol

CPA2

UniProt

P48052

Additional Carboxypeptidase A2/CPA2 Products

Product Documents for Human Carboxypeptidase A2/CPA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Carboxypeptidase A2/CPA2 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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