|Carnosine Dipeptidase 1/CNDP1 in Human Heart. Carnosine Dipeptidase 1/CNDP1 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Carnosine Dipeptidase 1/CNDP1 Monoclonal Antibody (Catalog # MAB2489) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
|Carnosine Dipeptidase 1/CNDP1 in BG01V Human Embryonic Stem Cells. Carnosine Dipeptidase 1/CNDP1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neurons using Mouse Anti-Human Carnosine Dipeptidase 1/CNDP1 Monoclonal Antibody (Catalog # MAB2489) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.|
The human CNDP1 gene encodes carnosine dipeptidase 1, a member of the M20 family of metalloproteases (1, 2). Also known as X-His dipeptidase, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), CNDP1 is a secreted dipeptidase with a narrow specificity for Xaa-His dipeptides including those with Xaa = beta -Ala (carnosine) and Xaa = gamma -aminobutyric acid (homocarnosine), two naturally occurring dipeptides with potential neuroprotective and neurotransmitter fucntions in the brain. In comparison, a closely related protein known as CNDP2, CPGL or CN2, is a cytosolic nonspecific dipeptidase.