Cathepsin D is a lysosomal aspartic protease of the pepsin family (1). Human cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (aa 1‑18), a propeptide (aa 19-64), and a mature chain (aa 65‑412) (2‑4). The mature chain can be processed further to the light (aa 65‑161) and heavy (aa 169‑412) chains. It is expressed in most cells and overexpressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Leu21-Leu412
Accession # P07339.1
Specificity
Clonality
Host
Isotype
Applications for Human Cathepsin D Antibody
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Cathepsin D Monoclonal Antibody (Catalog # MAB10142).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Reviewed Applications
Read 1 review rated 4 using MAB10143 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Cathepsin D
References
- Conner et al. in Handbook of Proteolytic Enzymes Barrett (1998) Academic Press, San Diego, p. 828.
- Faust, et al. (1985) Proc. Natl. Acad. Sci. USA 82:4910.
- Westley and May (1987) Nucl. Acid Res. 15:3773.
- Redecker, et al. (1991) DNA Cell Biol. 10:423.
- Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
- Tsukuba, et al. (2000) Mol. Cells 10:601.
Alternate Names
Gene Symbol
UniProt
Additional Cathepsin D Products
Product Documents for Human Cathepsin D Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Cathepsin D Antibody
For research use only
Customer Reviews for Human Cathepsin D Antibody (1)
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 06/17/2019This antibody was paired with MAB10142 in an immunoassay.
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Protocols
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Associated Pathways
