Human Cathepsin D Antibody

Catalog # Availability Size / Price Qty
AF1014
AF1014-SP
Human Cathepsin D Antibody in Western Blot
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Product Details
Citations (7)
FAQs
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Human Cathepsin D Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cathepsin D in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) Cathepsin D is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin D
Leu21-Leu412
Accession # P07339
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cathepsin D (Catalog # 1014-AS), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Cathepsin D by Western Blot. View Larger

Detection of Human Cathepsin D by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Procathepsin D at approximately 45 kDa and Cathepsin D heavy chain 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human Cathepsin D by Western Blot. View Larger

Detection of Human Cathepsin D by Western Blot. Western blot shows lysates of PANC-1 human pancreatic carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Procathepsin D at approximately 45 kDa and Cathepsin D heavy chain 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cathepsin D in Human Lung Cancer Tissue. View Larger

Cathepsin D in Human Lung Cancer Tissue. Cathepsin D was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin D

Cathepsin D is a lysosomal aspartic protease of the pepsin family (1). Human cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑18), a propeptide (residues 19-64), and a mature chain (residues 65‑412) (2‑4). The mature chain can be processed further to the light (residues 65‑161) and heavy (residues 169‑412) chains. It is expressed in most cells and overexpressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

References
  1. Conner et al. in Handbook of Proteolytic Enzymes Barrett (1998) Academic Press, San Diego, p. 828.
  2. Faust, et al. (1985) Proc. Natl. Acad. Sci. USA 82:4910.
  3. Westley and May (1987) Nucl. Acid Res. 15:3773.
  4. Redecker, et al. (1991) DNA Cell Biol. 10:423.
  5. Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
  6. Tsukuba, et al. (2000) Mol. Cells 10:601.
Entrez Gene IDs
1509 (Human); 13033 (Mouse)
Alternate Names
cathepsin D (lysosomal aspartyl protease); Cathepsin D; CPSD; CTSD; EC 3.4.23; EC 3.4.23.5; lysosomal aspartyl peptidase; lysosomal aspartyl protease; MGC2311; neuronal 10

Product Datasheets

Citations for Human Cathepsin D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Identification of PIKfyve kinase as a target in multiple myeloma
    Authors: C Bonolo De, YX Zhu, N Sepetov, S Romanov, LA Bruins, CX Shi, CK Stein, JL Petit, AN Polito, ME Sharik, EW Meermeier, GJ Ahmann, ID Lopez Arme, J Kruse, PL Bergsagel, M Chesi, N Meurice, E Braggio, AK Stewart
    Haematologica, 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Visualizing the cellular route of entry of a cystine-knot peptide with Xfect transfection reagent by electron microscopy
    Authors: X Gao, A De Mazière, DB Iaea, CP Arthur, J Klumperman, C Ciferri, RN Hannoush
    Sci Rep, 2019;9(1):6907.
    Species: Human
    Sample Types: Whole Cells
    Applications: Electron Microscopy
  3. HSP90 inhibition targets autophagy and induces a CASP9-dependent resistance mechanism in NSCLC
    Authors: J Han, LA Goldstein, W Hou, S Chatterjee, TF Burns, H Rabinowich
    Autophagy, 2018;0(0):1-14.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Amphiphilic star PEG-Camptothecin conjugates for intracellular targeting
    J Control Release, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  5. The Apaf-1-binding protein Aven is cleaved by Cathepsin D to unleash its anti-apoptotic potential.
    Cell Death Differ., 2012;19(9):1435-45.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. IFN-gamma regulation of vacuolar pH, cathepsin D processing and autophagy in mammary epithelial cells.
    Authors: Khalkhali-Ellis Z, Abbott DE, Bailey CM, Goossens W, Margaryan NV, Gluck SL, Reuveni M, Hendrix MJ
    J. Cell. Biochem., 2008;105(1):208-18.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Clathrin is a key regulator of basolateral polarity.
    Authors: Deborde S, Perret E, Gravotta D, Deora A, Salvarezza S, Schreiner R, Rodriguez-Boulan E
    Nature, 2008;452(7188):719-23.
    Species: Canine
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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