Detects human Cathepsin V in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Cathepsin C is observed and less than 5% cross-reactivity with rhCathepsin A, rhCathepsin D, rhCathepsin X/Z/P, rhCathepsin S, and rhCathepsin L is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human Cathepsin V Val18-Val334 Accession # O60911
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Measured by its ability to neutralize Recombinant Human Cathepsin V (0.5 µg/mL, Catalog # 1080-CY) cleavage of the fluorogenic peptide substrate Z-LR-AMC (10 µM, Catalog # ES008). The Neutralization Dose (ND50) is typically 2.2 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cathepsin V in Human Breast Cancer Tissue. Cathepsin V was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 1.7 µg/mL Goat Anti-Human Cathepsin V Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1080) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin V
Cathepsin V (also known as Cathepsin L2 and U) is a cysteine protease of the papain family (1). It is expressed as a proenzyme in lysosomes, which can be autocatalytically converted to the mature form at pH 4.0 (2). Cathepsin V shows high amino acid identity with Cathepsin L (77% for the proenzymes and 80% for the mature enzymes) (3). However, Cathepsin V is specifically expressed in the thymus, testis, and corneal epithelium whereas Cathepsin L is expressed throughout the body.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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