Human CCL13/MCP-4 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL13/MCP‑4 in ELISAs. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) CCL2 is observed and no significant cross-reactivity or interference with rhCCL5, 7, 8, 11, 24, recombinant mouse CCL2, 5, 7, 11 or 12 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 73506
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CCL13/MCP-4
    Gln24-Thr98
    Accession # Q99616.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    A549 human lung carcinoma cell line treated with recombinant human IL-1 beta, fixed with paraformaldehyde and permeabilized with saponin
    • Human CCL13/MCP-4 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human CCL13/MCP‑4 Antibody (Catalog # MAB327)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human CCL13/MCP‑4 Biotinylated Antibody (Catalog # BAF327)
  • ELISA Standard
     
    Recombinant Human CCL13/MCP-4 Protein (Catalog # 327-P4)
  • Neutralization
    Measured by its ability to neutralize CCL13/MCP‑4-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 2 µg/mL Recombinant Human CCL13/MCP‑4.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL13/MCP‑4 and Neutral­ization by Human CCL13/
MCP‑4 Antibody.
Recombinant Human CCL13/
MCP‑4 (Catalog # 327‑P4) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL13/MCP‑4 (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Human CCL13/
MCP‑4 Monoclonal Antibody (Catalog # MAB327). The ND50 is typically 10‑30 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL13/MCP-4

CCL13, also known as Monocyte Chemoattractant Protein-4 (MCP-4), is a CC chemokine that acts as a chemoattractant for monocytes, eosinophils and T cells and as an activator of basophils. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. The bioactivities of CCL13 are mediated by the CC chemokine receptors CCR-2 and CCR-3.

  • Entrez Gene IDs:
    6357 (Human)
  • Alternate Names:
    C-C motif chemokine 13; CCL13; chemokine (C-C motif) ligand 13; CKb10; MCP4; MCP-4; MCP-4Monocyte chemoattractant protein 4; MGC17134; NCC-1Monocyte chemotactic protein 4; NCC1Small-inducible cytokine A13; new CC chemokine 1; SCYA13CK-beta-10; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Type: Blister Fluid
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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