Human CCL13/MCP-4 Antibody

Catalog # Availability Size / Price Qty
AF327
AF327-SP
Chemotaxis Induced by CCL13/MCP‑4 and Neutralization by Human CCL13/MCP‑4 Antibody.
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Product Details
Citations (3)
FAQs
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Human CCL13/MCP-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL13/MCP-4 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL13/MCP-4
Gln24-Thr98
Accession # Q99616
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL13/MCP‑4 (Catalog # 327-P4)
Neutralization
Measured by its ability to neutralize CCL13/MCP‑4-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 15-40 µg/mL in the presence of 2 µg/mL Recombinant Human CCL13/MCP‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL13/MCP‑4 and Neutralization by Human CCL13/MCP‑4 Antibody. View Larger

Chemotaxis Induced by CCL13/MCP‑4 and Neutralization by Human CCL13/MCP‑4 Antibody. Recombinant Human CCL13/MCP-4 (Catalog # 327-P4) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CCL13/MCP-4 (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL13/MCP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF327). The ND50 is typically 15-40 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL13/MCP-4

Human CCL13 is a CC chemokine recently cloned from a human fetal and a human heart cDNA library. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4+ and CD8+ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR-2 and CCR-3, both of which have been shown to bind CCL13.

Entrez Gene IDs
6357 (Human)
Alternate Names
C-C motif chemokine 13; CCL13; chemokine (C-C motif) ligand 13; CKb10; MCP4; MCP-4; MCP-4Monocyte chemoattractant protein 4; MGC17134; NCC-1Monocyte chemotactic protein 4; NCC1Small-inducible cytokine A13; new CC chemokine 1; SCYA13CK-beta-10; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13

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Citations for Human CCL13/MCP-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. CCL13 is a promising diagnostic marker for systemic sclerosis.
    Authors: Yanaba K, Yoshizaki A, Muroi E, Hara T, Ogawa F, Shimizu K, Hasegawa M, Fujimoto M, Takehara K, Sato S
    Br. J. Dermatol., 2010;162(2):332-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Sequential involvement of CCR2 and CCR6 ligands for immature dendritic cell recruitment: possible role at inflamed epithelial surfaces.
    Authors: Vanbervliet B, Homey B, Durand I, Massacrier C, Ait-Yahia S, de Bouteiller O, Vicari A, Caux C
    Eur J Immunol, 2002;32(1):231-42.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Common and differential chemokine expression patterns in rs cells of NLP, EBV positive and negative classical Hodgkin lymphomas.
    Authors: Maggio EM, Van Den Berg A, Visser L, Diepstra A, Kluiver J, Emmens R, Poppema S
    Int. J. Cancer, 2002;99(5):665-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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