Human CCL15/MIP-1δ Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL15/MIP‑1δ in direct ELISAs and Western blots. In direct ELISAs and Western blots, a preference for the 68 aa residue isoform over the 92 aa residue isoform of human CCL15/LKN-1 is shown. Additionally, in direct ELISAs, less than 1% cross-reactivity with with rh6Ckine, rhBLC/BCA1, rhENA78, rhEotaxin, rhEotaxin2, rhFractalkine, rhGCP2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC1, rhHCC4, rhI309,rhIL8, rhMCP1, rhMCP2, rhMCP3, rhMCP4, rhMDC, rhMIG, rhMIP1 alpha, rhMIP1 beta, rhMIP3 alpha, rhMIP3 beta, rhMPIF1, rhNAP2, rhPARC, rhRANTES, rhSDF1 alpha,rhSDF1 beta,rhTarc, rhTECK, and rhVIC is observed. Neutralizes the biological activity of the 68 aa isoform of recombinant human (rh) CCL15/LKN-1. It will also neutralize the biological activity of the 92 aa residue isoform of rhCCL15/LKN-1 using a 2-fold higher Ig concentration.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CCL15/MIP-1δ
    Ser46-Ile113
    Accession # Q16663
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human CCL15/MIP‑1δ 68 aa (Catalog # 628-LK)
  • Neutralization
    Measured by its ability to neutralize CCL15/MIP‑1δ-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 15 ng/mL Recombinant Human CCL15/MIP‑1δ 68 aa.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL15/MIP‑1δ and Neutral­ization by Human CCL15/MIP‑1δ Antibody. Recombinant Human CCL15/MIP‑1δ (Catalog # 628‑LK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/MIP‑1δ (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL15/MIP‑1δ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF628). The ND50 is typically 0.5-2.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL15/MIP-1 delta

CCL15, also named Leukotactin-1 (LKN-1), MIP-5, HCC-2, and NCC-3, is a novel human CC chemokine whose gene was mapped to human chromosome 17 adjacent to the HCC-1 gene. CCL15/LKN-1, together with mouse C10, mouse MIP-1 gamma  and human MPIF-1, constitute a subgroup of CC chemokines which contain six instead of four conserved cysteine residues. The two additional cysteine residues in CCL15/LKN-1 have been shown to form a third disulfide bond CCL15/LKN-1 cDNA encodes a 113 amino acid (aa) residue precursor protein with a putative signal peptide of 21 aa residues that is cleaved to generate a 92 aa residue mature protein. In recombinant CCL15/LKN-1 preparations produced in insect cells and in yeast, amino-terminal truncations were found to have occurred. The major forms of CCL15/LKN-1 secreted by insect cells and yeast were reported to be proteins of 68 and 66 aa residues, respectively. The full length and the amino-terminal truncated forms of human CCL15δ/LKN-1 have been shown to be potent chemoattractants for monocytes and T-lymphocytes. These proteins can also chemoattract eosinophils and have been shown to induce calcium flux in human CCR1 transfected cells. Additionally, CCL15/LKN-1 can suppress colony formation by human granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors.

  • References:
    1. Youn, B.-S. et al. (1997) J. Immunol. 159:5201.
    2. Pardigol, A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6308.
    3. Wang, W. et al. (1998) J. Clinical Immunol.18:214.
    4. Coulin, F. et al. (1997) Eur. J. Biochem. 248:507.
  • Entrez Gene IDs:
    6359 (Human)
  • Alternate Names:
    CCL15; chemokine (C-C motif) ligand 15; Chemokine CC-2; HCC-2; HCC-2Lkn-1; Leukotactin 1; Leukotactin-1; LKN1; LKN-1; Macrophage inflammatory protein 5; MIP1 delta; MIP-1 delta; MIP5; MIP-5; MIP-5MIP-1d; Mrp-2b; NCC-3; NCC-3MIP-1D; NCC3MRP-2B; new CC chemokine 3; SCYA15member 15; Small-inducible cytokine A15
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