Human CCL16/HCC-4 Antibody Summary
Accession # O15467
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL16/HCC‑4 and Neutralization by Human CCL16/HCC‑4 Antibody.
Recombinant Human CCL16/HCC‑4 (Catalog # 802‑HC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL16/HCC‑4 (750 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL16/HCC‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF802). The ND50 is typically 4‑16 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% aa sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, gamma δ T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10. The HCC-4 gene has been mapped to chromosome 17q where multiple CC chemokines are clustered.
Recombinant HCC-4 has been shown to chemoattract human monocytes and THP-1 cells but not resting lymphocytes or neutrophils. HCC-4 has also been found to suppress proliferation of myeloid progenitor cells. The HCC-4 induced calcium flux in THP-1 cells can be desensitized by prior exposure to RANTES, suggesting that HCC-4 and RANTES share the same receptor in THP-1 cells.
- Shoudai, K. et al. (1998) Biochim. Biophys. Acta 1396:273.
- Hedrick, J. et al. (1998) Blood 91:4242.
- Youn, B-S. et al. (1998) BBRC 247:217.
Citations for Human CCL16/HCC-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
Authors: Haringman JJ, Smeets TJ, Reinders-Blankert P
Ann. Rheum. Dis., 2006;65(3):294-300.
Sample Types: Whole Tissue
An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
Authors: Rump L, Mattey D, Kehoe O, Middleton J
Sample Types: Whole Tissue
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