Human CCL2/JE/MCP-1 Antibody

Catalog # Availability Size / Price Qty
Detection of Recombinant Human CCL2/JE/MCP‑1 by Western Blot.
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Product Details
Citations (11)
Supplemental Products
Reviews (4)

Human CCL2/JE/MCP-1 Antibody Summary

Species Reactivity
Detects human CCL2/JE/MCP-1 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human CCL2/JE/MCP-1
Accession # P13500
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
See below
10-25 µg/mL
See below
Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 1.5-4.5 µg/mL in the presence of 150 ng/mL Recombinant Human CCL2/JE/MCP‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human CCL2/JE/MCP-1 antibody by Western Blot. View Larger

Detection of Recombinant Human CCL2/JE/MCP‑1 by Western Blot. Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Human CCL8/MCP-2 (Catalog # 281-CP), and Recombinant Human CCL13/MCP-4 (Catalog # 327-P4). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry CCL2/JE/MCP-1 antibody in Human Crohn's Disease Intestine by Immunohistochemistry (IHC-P). View Larger

CCL2/JE/MCP‑1 in Human Crohn's Disease Intestine. CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Goat Anti-Human CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) at 25 µg/mL for overnight at 4° C temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization Chemotaxis Induced by CCL2/MCP‑1 and Neutral-ization by Human CCL2/ MCP‑1 Antibody. View Larger

Chemotaxis Induced by CCL2/MCP‑1 and Neutral-ization by Human CCL2/ MCP‑1 Antibody. Recombinant Human CCL2/ MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL2/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA). The ND50 is typically 1.5-4.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL2/JE/MCP-1

CCL2, also known as monocyte chemotactic and activating factor (MCAF), was initially purified independently by two groups based on its ability to chemoattract monocytes. The human CCL2 cDNA encodes a 99 amino acid residue precursor protein with a 23 residue hydrophobic signal peptide that is cleaved to generate the 76 residue mature protein. Natural CCL2 is heterogeneous in size due to the addition of O-linked carbohydrates and sialic acid residues. In addition to fibroblasts; tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can also produce CCL2 either constitutively or upon stimulation by various stimuli. CCL2 is a member of the  beta (C-C) subfamily of chemokines. The existence of MCP-2 and MCP-3 with 62% and 73% amino acid identity respectively, to CCL2 have been reported. Consistent with it being a member of the chemokine beta family, CCL2 has been shown to chemoattract monocytes. In addition, it will also activate monocytes to be cytostatic for some human tumor cell lines; to increase cytosolic free calcium; to generate and release monocyte superoxide anions and to release monocyte lysosomal enzymes in vitro. CCL2 was reported to be capable of regulating adhesion molecule expression and cytokine production in human monocytes as well as chemoattracting, activating, and inducing histamine release from basophils. The biological roles played by CCL2 in a number of inflammatory and non-inflammatory disease states characterized by the accumulation of leukocytes at the site of the lesion, including atherosclerosis, delayed hypersensitivity reactions, etc., are being determined. CCL2 can bind to the C-C chemokine receptor-1 that also binds MIP-1 alpha, RANTES and MIP-1 beta. A specific receptor for CCL2 has also been cloned from THP-1 and MonoMac 6 cells.

Entrez Gene IDs
6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

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Citations for Human CCL2/JE/MCP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Teneligliptin prevents doxorubicin-induced inflammation and apoptosis in H9c2 cells
    Authors: W Peng, D Rao, M Zhang, Y Shi, J Wu, G Nie, Q Xia
    Arch. Biochem. Biophys., 2019;0(0):108238.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
    Authors: Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, Liu Z
    Autophagy, 2014;10(2):257-68.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Fractalkine is a novel human adipochemokine associated with type 2 diabetes.
    Authors: Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP
    Diabetes, 2011;60(5):1512-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype.
    Authors: Ouwehand K, Oosterhoff D, Breetveld M
    J. Invest. Dermatol., 2011;131(2):418-25.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Neutralization
  5. Interleukin-18 predicts atherosclerosis progression in SIV-infected and uninfected rhesus monkeys (Macaca mulatta) on a high-fat/high-cholesterol diet.
    Authors: Yearley JH, Xia D, Pearson CB, Carville A, Shannon RP, Mansfield KG
    Lab. Invest., 2009;89(6):657-67.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression.
    Authors: Fujimoto H, Sangai T, Ishii G, Ikehara A, Nagashima T, Miyazaki M, Ochiai A
    Int. J. Cancer, 2009;125(6):1276-84.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
    Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
    Arthritis Rheum., 2008;58(11):3510-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
    Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
    J. Neurosci., 2008;28(13):3277-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.
    Authors: Metelitsa LS, Wu HW, Wang H, Yang Y, Warsi Z, Asgharzadeh S, Groshen S, Wilson SB, Seeger RC
    J. Exp. Med., 2004;199(9):1213-21.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. Inhibition of neointimal hyperplasia after balloon injury by cis-element 'decoy' of early growth response gene-1 in hypercholesterolemic rabbits.
    Authors: Ohtani K, Egashira K, Usui M, Ishibashi M, Hiasa KI, Zhao Q, Aoki M, Kaneda Y, Morishita R, Takeshita A
    Gene Ther., 2004;11(2):126-32.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Alterations in expression of monocyte chemotactic protein-1 in the simian immunodeficiency virus model of disseminated Mycobacterium avium complex.
    Authors: Hendricks EE, Lin KC, Boisvert K, Pauley D, Mansfield KG
    J. Infect. Dis., 2004;189(9):1714-20.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P


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Goat IgG (H+L) Antibody


Reviews for Human CCL2/JE/MCP-1 Antibody

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Human CCL2/JE/MCP-1 Antibody
By Zoia Levashova on 11/20/2018
Application: MSD assay Sample Tested: Vitreous humor Species: Cynomolgus Monkey and Porcine

After labeling with Sulfo-Tag, used as a detection reagent in MSD assay (Meso Scale Diagnostics LLC).
A: Paired with biotinylated anti-human CCL2 antibody (MAB279) as a capture reagent. Calibration curve with Recombinant Human CCL2 (279-MC-010/CF) is shown (dynamic range 6-25,000 pg/ml)
B: Paired with biotinylated anti-porcine CCL2 antibody (Abcam, Cat# ab193793). Calibration curve with recombinant swine CCL2 (ImmunoChemistry Technologies, Cat# 6374) is shown (dynamic range 6-25,000 pg/ml).

Human CCL2/JE/MCP-1 Antibody
By Alisha Freeman on 12/13/2017
Application: WB Sample Tested: CHO Chinese hamster ovary cell line Species: Hamster

Human CCL2/JE/MCP-1 Antibody
By Balaji Mahender on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human CCL2/JE/MCP-1 Antibody
By Michelle Chen on 05/10/2017
Application: B/N Sample Tested: NHDF human normal dermal fibroblasts Species: Human