Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat, Primate - Macaca mulatta (Rhesus Macaque), Rabbit

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, Bioassay, ELISA Detection

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CCL2/JE/MCP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL2/JE/MCP-1
Gln24-Thr99
Accession # P13500

Specificity

Detects human CCL2/JE/MCP-1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL2/JE/MCP‑1 Antibody

Detection of Recombinant Human CCL2/JE/MCP-1 antibody by Western Blot.

Detection of Recombinant Human CCL2/JE/MCP‑1 by Western Blot.

Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Human CCL8/MCP-2 (Catalog # 281-CP), and Recombinant Human CCL13/MCP-4 (Catalog # 327-P4). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
CCL2/JE/MCP-1 antibody in Human Crohn's Disease Intestine by Immunohistochemistry (IHC-P).

CCL2/JE/MCP‑1 in Human Crohn's Disease Intestine.

CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Goat Anti-Human CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) at 25 µg/mL for overnight at 4° C temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Chemotaxis Induced by CCL2/MCP‑1 and Neutral-ization by Human CCL2/ MCP‑1 Antibody.

Chemotaxis Induced by CCL2/MCP‑1 and Neutral-ization by Human CCL2/ MCP‑1 Antibody.

Recombinant Human CCL2/ MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL2/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA). The ND50 is typically 1.5-4.5 µg/mL.

Applications for Human CCL2/JE/MCP‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

10-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Crohn's disease intestine

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC)

Neutralization

Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 1.5-4.5 µg/mL in the presence of 150 ng/mL Recombinant Human CCL2/JE/MCP‑1.

Reviewed Applications

Read 4 reviews rated 4.8 using AF-279-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL2/JE/MCP-1

CCL2, also known as monocyte chemotactic and activating factor (MCAF), was initially purified independently by two groups based on its ability to chemoattract monocytes. The human CCL2 cDNA encodes a 99 amino acid residue precursor protein with a 23 residue hydrophobic signal peptide that is cleaved to generate the 76 residue mature protein. Natural CCL2 is heterogeneous in size due to the addition of O-linked carbohydrates and sialic acid residues. In addition to fibroblasts; tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can also produce CCL2 either constitutively or upon stimulation by various stimuli. CCL2 is a member of the  beta (C-C) subfamily of chemokines. The existence of MCP-2 and MCP-3 with 62% and 73% amino acid identity respectively, to CCL2 have been reported. Consistent with it being a member of the chemokine beta family, CCL2 has been shown to chemoattract monocytes. In addition, it will also activate monocytes to be cytostatic for some human tumor cell lines; to increase cytosolic free calcium; to generate and release monocyte superoxide anions and to release monocyte lysosomal enzymes in vitro. CCL2 was reported to be capable of regulating adhesion molecule expression and cytokine production in human monocytes as well as chemoattracting, activating, and inducing histamine release from basophils. The biological roles played by CCL2 in a number of inflammatory and non-inflammatory disease states characterized by the accumulation of leukocytes at the site of the lesion, including atherosclerosis, delayed hypersensitivity reactions, etc., are being determined. CCL2 can bind to the C-C chemokine receptor-1 that also binds MIP-1 alpha, RANTES and MIP-1 beta. A specific receptor for CCL2 has also been cloned from THP-1 and MonoMac 6 cells.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Gene Symbol

CCL2

UniProt

P13500

Additional CCL2/JE/MCP-1 Products

Product Documents for Human CCL2/JE/MCP‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL2/JE/MCP‑1 Antibody

For research use only

Citations for Human CCL2/JE/MCP‑1 Antibody

Customer Reviews for Human CCL2/JE/MCP‑1 Antibody (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human CCL2/JE/MCP-1 Antibody
    Name: Zoia Levashova
    Application: MSD assay
    Sample Tested: Vitreous humor
    Species: Cynomolgus Monkey and Porcine
    Verified Customer | Posted 11/20/2018
    After labeling with Sulfo-Tag, used as a detection reagent in MSD assay (Meso Scale Diagnostics LLC). A: Paired with biotinylated anti-human CCL2 antibody (MAB279) as a capture reagent. Calibration curve with Recombinant Human CCL2 (279-MC-010/CF) is shown (dynamic range 6-25,000 pg/ml) B: Paired with biotinylated anti-porcine CCL2 antibody (Abcam, Cat# ab193793). Calibration curve with recombinant swine CCL2 (ImmunoChemistry Technologies, Cat# 6374) is shown (dynamic range 6-25,000 pg/ml).
    Human CCL2/JE/MCP‑1 Antibody AF-279-NA
  • Human CCL2/JE/MCP-1 Antibody
    Name: Alisha Freeman
    Application: Western Blot
    Sample Tested: CHO Chinese hamster ovary cell line
    Species: Hamster
    Verified Customer | Posted 12/13/2017
  • Human CCL2/JE/MCP-1 Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2017
  • Human CCL2/JE/MCP-1 Antibody
    Name: Michelle Chen
    Application: Block/Neutralize
    Sample Tested: NHDF human normal dermal fibroblasts
    Species: Human
    Verified Customer | Posted 05/10/2017

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