Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Neutralization

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 52513
View all CCL25/TECK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL25/TECK
Gln24-Leu150
Accession # AAB69981

Specificity

Detects human CCL25/TECK in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7,  9/10/MIP-1 gamma, 11, 12, 17, 19, 21, 22, or recombinant rat CCL20.  is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL25/TECK Antibody

Chemotaxis Induced by CCL25/TECK and Neutral-ization by Human CCL25/TECK Antibody.

Chemotaxis Induced by CCL25/TECK and Neutral-ization by Human CCL25/TECK Antibody.

Recombinant Human CCL25/TECK (Catalog # 334-TK) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL25/TECK (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL25/ TECK Monoclonal Antibody (Catalog # MAB334). The ND50 is typically 3-12 µg/mL.

Applications for Human CCL25/TECK Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize CCL25/TECK-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR9. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 1.5 µg/mL Recombinant Human CCL25/TECK.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL25/TECK

CCL25 (thymus-expressed chemokine) is a CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 aa precursor protein with a 23 aa signal peptide that is cleaved to yield a 127 aa mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 aa protein that exhibits 49% aa sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 are chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 is also a specific agonist for the CCR9 chemokine receptor

References

  1. Vicari, A.P. et al. (1997) Immunity 7:291.

Alternate Names

TECK

Entrez Gene IDs

6370 (Human); 20300 (Mouse)

Gene Symbol

CCL25

UniProt

AAB69981

Additional CCL25/TECK Products

Product Documents for Human CCL25/TECK Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL25/TECK Antibody

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Human CCL25/TECK Antibody

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