Human CCL7/MCP-3/MARC Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL7/MCP‑3/MARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 6% cross-reactivity with recombinant human (rh) MCP-2 and rhMCP-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Protein A or G purified
  • Immunogen
    E. coli-derived recombinant human CCL7/MCP‑3/MARC
    Gln24-109
    Accession # P80098
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CCL7/MCP‑3/MARC (Catalog # 282-P3)
  • Neutralization
    Measured by its ability to neutralize CCL7/MCP‑3/MARC-induced chemotaxis in human monocytes. The Neutralization Dose (ND50) is typically 30-60 µg/mL in the presence of 0.1 µg/mL Recombinant Human CCL7/MCP‑3/MARC.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL7/MCP‑3 and Neutral­ization by Human CCL7/MCP‑3 Antibody. Recombinant Human CCL7/
MCP‑3 (Catalog # 282‑P3) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL7/MCP‑3 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL7/
MCP‑3 Polyclonal Antibody (Catalog # AB-282-NA). The ND50 is typically 30-60 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 1 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL7/MCP-3/MARC

MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.

CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.

Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

  • Entrez Gene IDs:
    6354 (Human); 20306 (Mouse)
  • Alternate Names:
    C-C motif chemokine 7; CCL7/MCP-3/MARC; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Let-7d suppresses growth, metastasis, and tumor macrophage infiltration in renal cell carcinoma by targeting COL3A1 and CCL7.
    Authors: Su, Boxing, Zhao, Wei, Shi, Bentao, Zhang, Zhongyua, Yu, Xi, Xie, Feng, Guo, Zhongqia, Zhang, Xiaoyu, Liu, Jin, Shen, Qi, Wang, Jinghua, Li, Xuesong, Zhang, Zhiqian, Zhou, Liqun
    Mol Cancer, 2014;13(0):206.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 138  
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB, Simple Western HAF109 13  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF017 11  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 6
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 2  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 4  
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 3  
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 4
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Donkey Anti-Goat IgG Antibody

WB AF109 5
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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