Detects human CCL7/MCP‑3/MARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 6% cross-reactivity with recombinant human (rh) MCP-2 and rhMCP-1 is observed.
Polyclonal Goat IgG
Protein A or G purified
E. coli-derived recombinant human CCL7/MCP‑3/MARC Gln24-109 Accession # P80098
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human CCL7/MCP‑3/MARC (Catalog # 282-P3)
Measured by its ability to neutralize CCL7/MCP‑3/MARC-induced chemotaxis in human monocytes. The Neutralization Dose (ND50) is typically 30-60 µg/mL in the presence of 0.1 µg/mL Recombinant Human CCL7/MCP‑3/MARC.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL7/MCP‑3 and Neutralization by Human CCL7/MCP‑3 Antibody. Recombinant Human CCL7/ MCP‑3 (Catalog # 282‑P3) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL7/MCP‑3 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL7/ MCP‑3 Polyclonal Antibody (Catalog # AB-282-NA). The ND50 is typically 30-60 µg/mL.
Preparation and Storage
Reconstitute at 1 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.
CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.
Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Entrez Gene IDs:
6354 (Human); 20306 (Mouse)
C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)
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