Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 152211
View all CCRL2/CRAM-A/B Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CCRL2/CRAM‑A/B
Met1-Val356 (Val180Met)
Accession # NP_001124382

Specificity

Detects human CCRL2/CRAM‑A/B. Stains human HCR-transfected cells but not irrelevant transfectants. It detects both the CRAM-A and CRAM-B isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human CCRL2/CRAM‑A/B Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human CCRL2/CRAM-A transfected NS0 cells

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCRL2/CRAM-A/B

HCR, also known as CRAM-A, CRAM-B, CKRX and CCRL2, is a seven-transmembrane G-protein linked receptor that shares homology with other human chemokine receptors. Two isoforms were reported and designated CRAM-A and CRAM-B (1) that differ at their N-termini by the inclusion of an additional 12 amino acids on CRAM-A. HCR/CRAM is expressed at varying levels on a variety of peripheral blood cells including monocytes, neutrophils and T cells (2, 3).

References

  1. Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.
  2. Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.

Long Name

CC Chemokine Receptor-like 2

Alternate Names

ACKR5, CCRL2, CKRX, CRAM-A, CRAM-B, CRAMA, CRAMB, HCR

Entrez Gene IDs

9034 (Human)

Gene Symbol

CCRL2

UniProt

NP_001124382

Additional CCRL2/CRAM-A/B Products

Product Documents for Human CCRL2/CRAM‑A/B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCRL2/CRAM‑A/B Antibody

For research use only

Citations for Human CCRL2/CRAM‑A/B Antibody

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