Human CD155/PVR Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CD155/PVR in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 300907
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD155/PVR
    Gly27-Asn343
    Accession # AAH15542
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CD155/PVR (Catalog # 2530-CD)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD155/PVR in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB25301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Detection of CD155/PVR in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB25301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
CD155/PVR in Human PBMCs. CD155/PVR was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB25301) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD155/PVR

CD155 [also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5)] is a 70 kDa type I transmembrane (TM) glycoprotein that is a member of the nectin-like (Necl) family of nectin-related molecules (1). Like nectins, Necl molecules are Ig superfamily members that contain three Ig-like extracellular domains, a TM segment, and a cytoplasmic tail. Unlike nectins, Necl molecules cannot interact with cytoplasmic afadin (1). While Nectins serve as cell adhesion molecules, the actual functions of most Necls are yet-to-be determined. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells (2, 3). The full-length (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N-terminal V-type and two C2-type Ig-like domains (2, 3). The V-type domain mediates polio virus binding (4). Three other isoforms exist, all of which retain the Ig-like domains. CD155δ is transmembrane with a shortened cytoplasmic tail of 25 aa. CD155 beta (352 aa) and CD155 gamma (344 aa) are 60‑65 kDa soluble forms that show removal of the TM segment and surrounding amino acids (2, 5). The soluble forms will bind the polio virus (due to the presence of the V-type Ig domain) but afford no protection against polio infection because of low circulating levels (5). CD155 has been demonstrated to bind vitronectin, nectin-3, and DNAM-1 (6‑8). DNAM-1 binding promotes monocyte migration and NK cell killing. CD155 is expressed in all normal tissues and is highly expressed in tumor cells of epithelial and neuronal origin.

  • References:
    1. Takai, Y. et al. (2003) Cancer Sci. 94:655.
    2. Mendelsohn, C.L. et al. (1989) Cell 56:855.
    3. Koike, H. et al. (1990) EMBO J. 9:3217.
    4. Koike, S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4104.
    5. Baury, B. et al. (2003) Biochem. Biophys. Res. Commun. 309:175.
    6. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
    7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
    8. Lange, R. et al. (2001) Virology 285:218.
  • Long Name:
    Poliovirus Receptor
  • Entrez Gene IDs:
    5817 (Human); 52118 (Mouse); 25066 (Rat)
  • Alternate Names:
    CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR; PVS; PVSFLJ25946; Tage4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Binding of the Fap2 protein of Fusobacterium nucleatum to human inhibitory receptor TIGIT protects tumors from immune cell attack.
    Authors: Gur C, Ibrahim Y, Isaacson B, Yamin R, Abed J, Gamliel M, Enk J, Bar-On Y, Stanietsky-Kaynan N, Coppenhagen-Glazer S, Shussman N, Almogy G, Cuapio A, Hofer E, Mevorach D, Tabib A, Ortenberg R, Markel G, Miklic K, Jonjic S, Brennan C, Garrett W, Bachrach G, Mandelboim O
    Immunity, 2015;42(2):344-55.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Not Specified
  2. Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR.
    Authors: Stanietsky N, Rovis T, Glasner A, Seidel E, Tsukerman P, Yamin R, Enk J, Jonjic S, Mandelboim O
    Eur J Immunol, 2013;43(8):2138-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
    Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S
    PLoS Pathog., 2012;8(1):e1002486.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44.
    Authors: Madrid, Alexis S, Ganem, Don
    J Virol, 2012;86(16):8693-704.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
    Authors: Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
    Oncogene, 2011;30(23):2622-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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