Human CD163 Antibody

(9 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD163 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD163 (R&D Systems, Catalog # 1607-CD)
    Gly41-Ser1045
    Accession # CAA80543
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CD163 by Western Blot. Western blot shows lysates of human thymus tissue, human liver tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD163 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human CD163 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for CD163 at approximately 179 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.        
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD163

CD163, previously called M130 or p155, is a 130-160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1-3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a
39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86%, and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7 - 10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL-10, IL-6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb-Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13-15).

  • References:
    1. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
    2. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
    3. Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
    4. Kristiansen, M. et al. (2001) Nature 409:198.
    5. Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
    6. Moller, H.J. et al. (2002) Blood 99:378.
    7. Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
    8. Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
    9. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
    10. Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
    11. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
    12. Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
    13. Schaer, D.J. et al. (2006) Blood 107:373.
    14. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
    15. Fabriek, B.O. et al. (2007) Blood 109:5223.
  • Entrez Gene IDs:
    9332 (Human); 93671 (Mouse); 312701 (Rat)
  • Alternate Names:
    CD163 antigenscavenger receptor cysteine-rich type 1 protein M130; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; M130macrophage-associated antigen; MM130; RM3/1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Applications
Sample Type
  1. Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model
    Oncotarget, 2016;7(21):31097-110.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Simian hemorrhagic fever virus cell entry is dependent on CD163 and uses a clathrin-mediated endocytosis-like pathway.
    Authors: Cai Y, Postnikova E, Bernbaum J, Yu S, Mazur S, Deiuliis N, Radoshitzky S, Lackemeyer M, McCluskey A, Robinson P, Haucke V, Wahl-Jensen V, Bailey A, Lauck M, Friedrich T, O'Connor D, Goldberg T, Jahrling P, Kuhn J
    J Virol, 2015;89(1):844-56.
    Species: Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Type: Whole Cells
    Application: Neut
  3. CD163 and IgG codefend against cytotoxic hemoglobin via autocrine and paracrine mechanisms.
    Authors: Subramanian K, Du R, Tan N, Ho B, Ding J
    J Immunol, 2013;190(10):5267-78.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Interaction of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) with sialoadhesin (CD169/Siglec-1) inhibits alveolar macrophage phagocytosis.
    Authors: De Baere MI, Van Gorp H, Delputte PL
    Vet. Res., 2012;43(1):47.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Neut
  5. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
    Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
    J Immunol, 2012;189(7):3508-20.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus.
    Authors: Van Gorp H, Van Breedam W, Van Doorsselaere J, Delputte PL, Nauwynck HJ
    J. Virol., 2010;84(6):3101-5.
    Species: Porcine
    Sample Type: Whole Cells
    Application: WB
  7. Sialoadhesin and CD163 join forces during entry of the porcine reproductive and respiratory syndrome virus.
    Authors: Van Gorp H, Van Breedam W, Delputte PL, Nauwynck HJ
    J. Gen. Virol., 2008;89(0):2943-53.
    Species: Porcine
    Sample Type: Whole Cells
    Application: ICC
  8. Availability of activated CD4+ T cells dictates the level of viremia in naturally SIV-infected sooty mangabeys.
    Authors: Klatt NR, Villinger F, Bostik P, Gordon SN, Pereira L, Engram JC, Mayne A, Dunham RM, Lawson B, Ratcliffe SJ, Sodora DL, Else J, Reimann K, Staprans SI, Haase AT, Estes JD, Silvestri G, Ansari AA
    J. Clin. Invest., 2008;118(6):2039-49.
    Species: Primate - Cercocebus torquatus (Sooty Mangabey)
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses.
    Authors: Calvert JG, Slade DE, Shields SL, Jolie R, Mannan RM, Ankenbauer RG, Welch SK
    J. Virol., 2007;81(14):7371-9.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Summary

ApplicationWestern Blot
Sample TestedCancer cell lysates
SpeciesHuman

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