Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 703335
View all CD1d Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD1d
Glu20-Ser301 (predicted)
Accession # P15813

Specificity

Detects human CD1d in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1c, rhCD1e, recombinant mouse (rm) CD1d1, and rmCD1d2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD1d Antibody

Detection of Human CD1d antibody by Western Blot.

Detection of Human CD1d by Western Blot.

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD1d Monoclonal Antibody (Catalog # MAB6979) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD1d at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human CD1d Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMC)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD1d

CD1d is a 48 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. Mature human CD1d consists of a 282 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. Within aa 19-300 (the ECD), human CD1d shares 65% and 68% aa sequence identity with mouse and rat CD1d, respectively. Complexes of CD1d with beta 2-microglobulin and endogenous glycolipids are constitutively expressed on antigen presenting cells, cortical thymocytes, liver sinusoidal endothelial cells, Kupffer cells, and hepatocytes (1). CD1d-presented glycolipids are recognized by canonical NKT cells that utilize an invariant mouse Va14-Ja18 chain in their T cell receptor (Va24-Ja18 in human) (2, 3). The interaction with glycolipid-loaded CD1d is critical for NKT cell development and induces their rapid secretion of both Th1 and Th2 type cytokines (3-6). In humans, infection with HSV-1 suppresses NKT cell activation by blocking the intracellular cycling of CD1d in antigen presenting cells (7).

References

  1. Bendelac, A. et al. (2007) Annu. Rev. Immunol. 25:297.
  2. Kawano, T. et al. (1997) Science 278:1626.
  3. Chiu, Y.H. et al. (1999) J. Exp. Med. 189:103.
  4. Behar, S.M. et al. (1999) J. Immunol. 162:161.
  5. Mendiratta, S.K. et al. (1997) Immunity 6:469.
  6. Stanic, A.K. et al. (2003) J. Immunol. 171:4539.
  7. Yuan, W. et al. (2006) Nat. Immunol. 7:835.

Alternate Names

CD1d, R3, R3G1

Entrez Gene IDs

912 (Human); 12480 (Mouse); 25109 (Rat)

Gene Symbol

CD1D

UniProt

P15813

Additional CD1d Products

Product Documents for Human CD1d Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD1d Antibody

For research use only

Citations for Human CD1d Antibody

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