Human CD39L4/ENTPD5 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD39L4/ENTPD5 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD39, rhCD39L1, or rhCD39L3 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 743512
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human CD39L4/ENTPD5
    Val21-His428
    Accession # O75356
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CD39L4/ENTPD5 by Western Blot. Western blot shows lysates of human liver tissue and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human CD39L4/ENTPD5 Monoclonal Antibody (Catalog # MAB5297) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD39L4/ENTPD5 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD39L4/ENTPD5

CD39L4, also called ENTPD5 (ectonucleoside triphosphate diphosphohydrolase 5), ER-UDPase and  PCPH, is a secreted, 45-51 kDa class II member of the GDA1/CD39 NTPase family of molecules. It is expressed by multiple cell types, including neurons, cardiomyocytes, and hepatocytes. CD39L4 is a divalent cation‑dependent enzyme that preferentially hydrolyzes GDP and UDP. In the ER, this enzyme may remove UDP which interferes with proper protein folding and maturation. Extracellularly, CD39L4 may reduce available UDP ligand for P2Y receptors. Mature human CD39L4 is a 408 amino acid (aa) glycoprotein (aa 21-428). It contains four APR (apyrase conserved regions) (aa 54-206) that participate in NTPase activity. CD39L4 has been reported to exist as both monomer and homodimer. Three potential splice variants are reported. One shows a seven aa substitution for aa 401-428, a second contains a five aa substitution for aa 401-428, and a third possesses a 22 aa substitution for aa 215-428. Over aa 21-428, human CD39L4 shares 89% aa sequence identity with mouse CD39L4.

  • Long Name:
    Ecto-Nucleoside Triphosphate Diphosphohydrolase 5
  • Entrez Gene IDs:
    957 (Human); 12499 (Mouse); 314312 (Rat)
  • Alternate Names:
    CD39 antigen-like 4; CD39L4; CD39L4NTPDase 5; CD39-like 4; EC 3.6.1; EC 3.6.1.42; EC 3.6.1.6; ectonucleoside triphosphate diphosphohydrolase 5; ENTPD5; ER-UDPase; GDPase ENTPD5; Guanosine-diphosphatase ENTPD5; MGC163357; MGC163359; NTPDase 5; NTPDase-5; Nucleoside diphosphatase; Pcph proto-oncogene protein; PCPH; proto-oncogene CPH; UDPase ENTPD5; Uridine-diphosphatase ENTPD5
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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