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Human CD4 Antibody

  • Specificity
    Detects human CD4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD4 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 34930
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4
    Extracellular domain
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood lymphocytes
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human lymph node
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CD4 in Human PBMCs. CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB379) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) precursor protein with a 25 aa signal peptide, a 371 aa extracellular region containing four immunoglobulin homology domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

  • References:
    1. Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.
  • Entrez Gene IDs:
    920 (Human); 24932 (Human); 403931 (Human); 12504 (Human); 493775 (Feline);
  • Alternate Names:
    CD4; CD4 antigen; CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 3 of 3
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Applications
Sample Type
  1. The transmembrane domain of the severe acute respiratory syndrome coronavirus ORF7b protein is necessary and sufficient for its retention in the Golgi complex.
    Authors: Schaecher SR, Diamond MS, Pekosz A
    J. Virol., 2008;82(19):9477-91.
    Species: Human
    Application: Western Blot,
  2. Presence of IgG-CD4 complexes in the circulation.
    Authors: Nezlin R, Bengtsson AA,
    Immunol. Invest., 2008;37(2):153-62.
    Species: Human
    Application: Dot Blot,
  3. Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity.
    Authors: Suzuki F, Kubota T
    Arthritis Res. Ther., 2012;14(2):R48.
    Species: Human
    Application: IHC Frozen,

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