Human CD4 Antibody
Human CD4 Antibody Summary
Extracellular domain
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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CD4 in Human PBMCs. CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB379) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD4
CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) precursor protein with a 25 aa signal peptide, a 371 aa extracellular region containing four immunoglobulin homology domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.
Product Datasheets
Citations for Human CD4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Infiltration and persistence of lymphocytes during late-stage cerebral ischemia in middle cerebral artery occlusion and photothrombotic stroke models
Authors: Y Feng, S Liao, C Wei, D Jia, K Wood, Q Liu, X Wang, FD Shi, WN Jin
J Neuroinflammation, 2017;14(1):248.
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Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma
Authors: P Ai, Z Li, Y Jiang, C Song, L Zhang, H Hu, T Wang
Oncol Lett, 2017;14(2):2458-2462.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Kv1.3 channels mark functionally competent CD8+ tumor infiltrating lymphocytes in head and neck cancer
Cancer Res., 2016;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity.
Authors: Suzuki F, Kubota T
Arthritis Res. Ther., 2012;14(2):R48.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Presence of IgG-CD4 complexes in the circulation.
Authors: Nezlin R, Bengtsson AA,
Immunol. Invest., 2008;37(2):153-62.
Species: Human
Sample Types: Serum
Applications: Dot Blot -
The transmembrane domain of the severe acute respiratory syndrome coronavirus ORF7b protein is necessary and sufficient for its retention in the Golgi complex.
Authors: Schaecher SR, Diamond MS, Pekosz A
J. Virol., 2008;82(19):9477-91.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Average Rating: 4 (Based on 2 Reviews)
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Immobilize Anti-CD4, flow dose response of CD4 (200-3nM. KD ~5nM
