Human CD46 Antibody

Detection of Human CD46 by Western Blot.

Western blot shows lysate of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD46 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2005) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD46 at approximately 50-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CD46 in Human Skin.

CD46 was detected in immersion fixed paraffin-embedded sections of human skin using 1.7 µg/mL Goat Anti-Human CD46 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2005) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of CD46 by Immunocytochemistry/ Immunofluorescence

Evaluation of the roles of CD46 and DSG2 in HAdV-7 infection using in vitro cell models. (A–E). Functional gain-of-function study in MG-63 cells transduced with Ad5-cd46 and/or Ad5-dsg2. The MG-63 cells were transfected with the empty Ad5 vector as the mock control group. Expression levels of hCD46 and hDSG2 were detected by RT-qPCR (A) and Western blot (B). Representative fluorescence microscopy images (C) and quantification (D) of HAdV7E infection at 48 hpi. Viral genome copy number was detected by qPCR following HAdV7E infection at 48 hpi (E). (F–J) Functional loss-of-function study in A549 cells transfected with siRNAs targeting CD46 and DSG2. Expression levels of hCD46 and hDSG2 were detected by RT-qPCR (F) and Western blot (G). Representative fluorescence microscopy images (H) and quantification (I) of HAdV7E infection at 48 hpi. Viral genome copy number was detected by qPCR following HAdV7E infection at 48 hpi (J). Human beta -actin was used as the internal reference in all experiments. A549 cells were transfected with scrambled siRNA serving as the NC. A549 cells without transfection were used as the mock group. Each experiment was repeated three times, with data presented as mean ± SD. Statistical significance was assessed by one-way ANOVA (n ≥ 3). *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41989164), licensed under a CC-BY license. Not internally tested by R&D Systems.