Human CD83 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CD83.
  • Source
    Monoclonal Mouse IgG1 Clone # HB15e
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    COS-7 African green monkey SV40 transformed kidney fibroblast-like cells transfected with human CD83
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD83 in Human Mature Dendritic Cells by Flow Cytometry. Human mature dendritic cells were stained with Mouse Anti-Human CD83 Monoclonal Antibody (Catalog # MAB1774, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
CD83 in Human Dendritic Cells. CD83 was detected in immersion fixed human dendritic cells using 10 µg/mL Mouse Anti-Human CD83 Monoclonal Antibody (Catalog # MAB1774) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunocytochemistry
CD83 in THP‑1 Human Cell Line. CD83 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Mouse Anti-Human CD83 Monoclonal Antibody (Catalog # MAB1774) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD83

Human CD83 is a 40‑50 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 125 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). Although in vitro studies suggest CD83 may form membrane-bound covalent homodimers, in vivo this does not appear to be the case (1, 4). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4, 5). Relative to human, mouse CD83 is 11 aa shorter in its extracellular domain and is expressed as a 30‑35 kDa protein (1, 4, 5). Human CD83 is active in the mouse system (4). One alternate splice form has been reported. This leads to a small monomeric soluble form of 74 aa that includes aa 20‑52 and aa 164‑205 (6, 7). In human, proteolytic cleavage and solubilization of CD83 has also been suggested, and this could lead to dimeric circulating CD83 (4, 6). CD83 is a primary marker for dendritic cells (3, 6, 8). It is also found on B cells (6, 9), neutrophils (10), monocytes and macrophages (11). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on target cells (12). Membrane CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (13, 14). Soluble CD83, however, appears to be immunosuppressive and blocks T cell activation (15, 16). On monocytes, CD83 is suggested to drive monocytes into a fibrocyte phenotype (13). A lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (17).

  • References:
    1. Zhou, L-J. et al. (1992) J. Immunol. 149:735.
    2. Kozlow, E.J. et al. (1993) Blood 81:454.
    3. Fujimoto, Y and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
    4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
    5. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
    6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
    7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
    8. Velten, F.W. et al. (2007) Mol. Immunol. 44:1544.
    9. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
    10. Yamashiro, S. et al. (2000) Blood 96:3958.
    11. Cao, W. et al. (2005) Biochem. J. 385:85.
    12. Scholler, N. et al. (2001) J. Immunol. 166:3865.
    13. Scholler, N. et al. (2002) J. Immunol. 168:2599.
    14. Hirano, N. et al. (2006) Blood 107:1528.
    15. Kotzor, N. et al. (2004) Immunobiology 209:129.
    16. Zinser, E. et al. (2006) Immunobiology 211:449.
    17. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.
  • Entrez Gene IDs:
    9308 (Human); 12522 (Mouse)
  • Alternate Names:
    B-cell activation protein; BL11; BL11CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 molecule; CD83; Cell surface protein HB15; cell-surface glycoprotein; HB15; HB15hCD83
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides.
    Authors: Izquierdo-Useros N, Lorizate M, Puertas M, Rodriguez-Plata M, Zangger N, Erikson E, Pino M, Erkizia I, Glass B, Clotet B, Keppler O, Telenti A, Krausslich H, Martinez-Picado J
    PLoS Biol, 2012;10(12):e1001448.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Glomerular accumulation of plasmacytoid dendritic cells in active lupus nephritis: role of interleukin-18.
    Authors: Tucci M, Quatraro C, Lombardi L, Pellegrino C, Dammacco F, Silvestris F
    Arthritis Rheum., 2008;58(1):251-62.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
    Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
    J. Pathol., 2008;214(3):328-36.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Interleukin-18 overexpression as a hallmark of the activity of autoimmune inflammatory myopathies.
    Authors: Tucci M, Quatraro C, Dammacco F, Silvestris F
    Clin. Exp. Immunol., 2006;146(1):21-31.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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