Human CD83 Antibody

Catalog # Availability Size / Price Qty
AF2044
AF2044-SP
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Human CD83 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD83 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse CD83 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD83
Thr20-Ala143
Accession # Q01151
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CD83 Fc Chimera (Catalog # 2044-CD)
Flow Cytometry
2.5 µg/106 cells
Human monocyte-derived mature dendritic cells treated with LPS, Recombinant Human TNF‑ alpha (Catalog # 210‑TA), and Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD83

Human CD83 is a 40 - 50 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1-3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 125 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). Although in vitro studies suggest CD83 may form membrane-bound covalent homodimers, in vivo this does not appear to be the case (1, 4). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4, 5). Relative to human, mouse CD83 is 11 aa shorter in its extracellular domain and is expressed as a 30-35 kDa protein (1, 4, 5). Human CD83 is active in the mouse system (4). One alternate splice form has been reported. This leads to a small monomeric soluble form of 74 aa that includes aa’s 20-52 and 164-205 (6, 7). In human, proteolytic cleavage and solubilization of CD83 has also been suggested, and this could lead to dimeric circulating CD83 (4, 6). CD83 is a primary marker for dendritic cells (3, 6, 8). It is also found on B cells (6, 9), neutrophils (10), monocytes, and macrophages (11). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on target cells (12). The function of CD83 is only now becoming clear. Membrane CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (13, 14). Soluble CD83, however, appears to be immunosuppressive and blocks T cell activation (15, 16). On monocytes, CD83 is suggested to drive monocytes into a fibrocyte phenotype (13). A lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (17).

References
  1. Zhou, L-J. et al. (1992) J. Immunol. 149:735.
  2. Kozlow, E.J. et al. (1993) Blood 81:454.
  3. Fujimoto, Y and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
  4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
  5. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
  6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
  7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
  8. Velten, F.W. et al. (2007) Mol. Immunol. 44:1544.
  9. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
  10. Yamashiro, S. et al. (2000) Blood 96:3958.
  11. Cao, W. et al. (2005) Biochem. J. 385:85.
  12. Scholler, N. et al. (2001) J. Immunol. 166:3865.
  13. Scholler, N. et al. (2002) J. Immunol. 168:2599.
  14. Hirano, N. et al. (2006) Blood 107:1528.
  15. Kotzor, N. et al. (2004) Immunobiology 209:129.
  16. Zinser, E. et al. (2006) Immunobiology 211:449.
  17. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.
Entrez Gene IDs
9308 (Human); 12522 (Mouse)
Alternate Names
B-cell activation protein; BL11; BL11CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 molecule; CD83; Cell surface protein HB15; cell-surface glycoprotein; HB15; HB15hCD83

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