Detection of CD98 in K562 Human Cell Line by Flow Cytometry.
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human CD98 Monoclonal Antibody (Catalog # MAB5920, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
CD98 in K562 Human Cell Line.
CD98 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human CD98 Monoclonal Antibody (Catalog # MAB5920) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD98, also known as 4F2, is a widely expressed disulfide-linked heterodimer in the solute transporter family. It consists of an 85 kDa glycosylated type II transmembrane heavy chain and a 40‑50 kDa non-glycosylated light chain with 12 transmembrane segments. The heavy chain (SLC3A2) pairs with one of several light chains (SLC7A5, 6, 7, 8, 10, or 11) and is required for the cell surface expression and amino acid transport function of the light chains. CD98 also mediates integrin signaling, T cell costimulation, B cell proliferation, and viral fusion with cell membranes. Within the extracellular region, human CD98 heavy chain shares 73% and 71% aa sequence identity with mouse and rat CD98 heavy chain, respectively.
Entrez Gene IDs:
6520 (Human); 17254 (Mouse)
4F2; 4F2hc; 4T2HC; antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC; CD98 antigen; CD98 heavy chain; CD98; CD98HC; CH98hc; FRP-1; heavy chain; Lymphocyte activation antigen 4F2 large subunit; MDU1antigen defined by monoclonal 4F2, heavy chain; monoclonal 44D7; NACAE4F2 cell-surface antigen heavy chain; SLC3A2; solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen
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