Human CELSR3 Antibody

Catalog # Availability Size / Price Qty
MAB7278
MAB7278-SP
Detection of Human CELSR3 by Western Blot.
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Human CELSR3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CELSR3 in ELISA.
Source
Monoclonal Mouse IgG2B Clone # 763103
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CELSR3
Val531-Ser711
Accession # Q9NYQ7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human CELSR3 antibody by Western Blot. View Larger

Detection of Human CELSR3 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human CELSR3. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human CELSR3 Monoclonal Antibody (Catalog # MAB7278) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CELSR3 at approximately 260 and 400 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry Detection of CELSR3 antibody in SH-SY5Y Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CELSR3 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human CELSR3 Monoclonal Antibody (Catalog # MAB7278, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Flow Cytometry Detection of CELSR3 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CELSR3 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Mouse Anti-Human CELSR3 Monoclonal Antibody (Catalog # MAB7278, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CELSR3

CELSR3 (Cadherin EGF LAG Seven-pass G-type Receptor 3; also cadherin family member 11/CDHF11, FMI1 and EGFL1) is a 355 kDa (predicted) member of the LN-7TM subfamily, GPCR 2 family of proteins. It is coexpressed with Fzd3 on postmigratory neurons of the developing DRG and cranial ganglia, and appears to serve as an axonal guidance cue. Mature human CELSR3 is 3280 amino acids (aa) in length (aa 33-3312). It is a highly complex 7-transmembrane protein that contains a 2508 aa extended N-terminal extracellular region (aa 33-2540) plus a 538 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 326-1265), followed by a mixture of eight EGF-like and three laminin-like domains, and one membrane-proximal GPS domain. There are two potential isoform variants. One shows a five aa insertion after Gly2158, while another possesses an alternative start site at Met276. Over aa 531-711, human CELSR3 shares 98% aa sequence identity with mouse CELSR3.

Long Name
Cadherin, EGF LAG Seven-pass G-type Receptor 3
Entrez Gene IDs
1951 (Human); 107934 (Mouse); 83466 (Rat)
Alternate Names
Cadherin family member 11; cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila); CDHF11; CDHF11RESDA1; CELSR3; EGFL1; EGFL1cadherin EGF LAG seven-pass G-type receptor 3; EGF-like protein 1; EGF-like-domain, multiple 1; epidermal growth factor-like 1; Epidermal growth factor-like protein 1; Flamingo homolog 1; FMI1anchor protein; HFMI1; KIAA0812; MEGF2; MEGF2cadherin EGF LAG seven-pass G-type receptor 3, flamingo (Drosophila) homolog; multiple EGF-like domains 2; Multiple EGF-like domains protein 2; Multiple epidermal growth factor-like domains protein 2; RESDA1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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