Detection of Human CELSR3 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human CELSR3. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human CELSR3 Monoclonal Antibody (Catalog # MAB7278) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CELSR3 at approximately 260 and 400 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of CELSR3 in SH‑SY5Y Human Cell Line by Flow Cytometry.
SH‑SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human CELSR3 Monoclonal Antibody (Catalog # MAB7278, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Detection of CELSR3 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Mouse Anti-Human CELSR3 Monoclonal Antibody (Catalog # MAB7278, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CELSR3 (Cadherin EGF LAG Seven-pass G-type Receptor 3; also cadherin family member 11/CDHF11, FMI1 and EGFL1) is a 355 kDa (predicted) member of the LN-7TM subfamily, GPCR 2 family of proteins. It is coexpressed with Fzd3 on postmigratory neurons of the developing DRG and cranial ganglia, and appears to serve as an axonal guidance cue. Mature human CELSR3 is 3280 amino acids (aa) in length (aa 33-3312). It is a highly complex 7-transmembrane protein that contains a 2508 aa extended N-terminal extracellular region (aa 33-2540) plus a 538 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 326-1265), followed by a mixture of eight EGF-like and three laminin-like domains, and one membrane-proximal GPS domain. There are two potential isoform variants. One shows a five aa insertion after Gly2158, while another possesses an alternative start site at Met276. Over aa 531-711, human CELSR3 shares 98% aa sequence identity with mouse CELSR3.
Cadherin, EGF LAG Seven-pass G-type Receptor 3
Entrez Gene IDs:
1951 (Human); 107934 (Mouse); 83466 (Rat)
Cadherin family member 11; cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila); CDHF11; CDHF11RESDA1; CELSR3; EGFL1; EGFL1cadherin EGF LAG seven-pass G-type receptor 3; EGF-like protein 1; EGF-like-domain, multiple 1; epidermal growth factor-like 1; Epidermal growth factor-like protein 1; Flamingo homolog 1; FMI1anchor protein; HFMI1; KIAA0812; MEGF2; MEGF2cadherin EGF LAG seven-pass G-type receptor 3, flamingo (Drosophila) homolog; multiple EGF-like domains 2; Multiple EGF-like domains protein 2; Multiple epidermal growth factor-like domains protein 2; RESDA1
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