Detection of Human Chitinase 3-like 2 by Western Blot.
Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Chitinase 3-like 2 Monoclonal Antibody (Catalog # MAB5116) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Chitinase 3-like 2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chitinase 3-like 2
Chitinase 3-like 2 (CHI3L2; also chrondrocyte protein 39 and YKL-39) is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family. It is secreted by synovial fibroblasts and chondrocytes, particularly in osteoarthritis, and may contribute to extracellular matrix stability. CHI3L2 has no chitotriosidase activity, but is likely to bind some type of glycan. The human CHI3L2 precursor is 390 amino acids (aa) in length. It contains a 26 aa signal sequence and a 364 aa mature region. There are two potential alternate start sites at Met24 and Met80; a third potential isoform shows a deletion of aa 15‑24. Over aa 27‑390, human CHI3L2 shares 89% aa identity with bovine CHI3L2. No rodent CHI3L2 has been reported.
Entrez Gene IDs:
CHI3L2; Chitinase 3 like 2; Chitinase 3-like 2; chitinase-3-like protein 2; Chondrocyte protein 39; YKL39; YKL-39YKL39
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.