Detection of Human Chk2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human Chk2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1358) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Chk2 was detected at approximately 65 kDa (as indicated). For additional reference, duplicate lysates were probed following incubation of the primary antibody with Recombinant Human Chk2 (right panel, Catalog # 1358‑KS). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Chk2 by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Chk2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Chk2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1358) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Chk2 at approximately 68 kDa (as indicated) in the parental HeLacell line, but is not detectable in knockout HeLa cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Serine/threonine-protein kinase Chk2 (CHEK2) is a 65 kDa member of Serine/threonine-protein kinase family of proteins. Chk2 regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Chk2 is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks.Existence of multiple splice variants of Chk2 is predicted. Human Chk2 shares 86% aa sequence identity with mouse Chk2.
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