Human Chk2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Chk2 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Chk2
    Asp208-Leu543
    Accession # O96017
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Knockout Validated
    Chk2 is specifically detected in HeLa humancervical epithelial carcinoma parental cell line but is not detectable inChk2 knockout HeLa cell line.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Chk2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human Chk2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1358) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Chk2 was detected at approx­imately 65 kDa (as indicated). For additional reference, duplicate lysates were probed following incubation of the primary antibody with Recombinant Human Chk2 (right panel, Catalog # 1358‑KS). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Knockout Validated
Western Blot Shows Human Chk2 by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Chk2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Chk2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1358) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Chk2 at approximately 68 kDa (as indicated) in the parental HeLacell line, but is not detectable in knockout HeLa cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chk2

Serine/threonine-protein kinase Chk2 (CHEK2) is a 65 kDa member of Serine/threonine-protein kinase family of proteins. Chk2 regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Chk2 is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks.Existence of multiple splice variants of Chk2 is predicted. Human Chk2 shares 86% aa sequence identity with mouse Chk2.

  • Long Name:
    Checkpoint Kinase 2
  • Entrez Gene IDs:
    11200 (Human); 50883 (Mouse); 114212 (Rat)
  • Alternate Names:
    CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); Chk2; EC 2.7.11; EC 2.7.11.1; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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