Serine/threonine-protein kinase Chk2 (CHEK2) is a 65 kDa member of Serine/threonine-protein kinase family of proteins. Chk2 regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Chk2 is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks.Existence of multiple splice variants of Chk2 is predicted. Human Chk2 shares 86% aa sequence identity with mouse Chk2.
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human Chk2
Asp208-Leu543
Accession # O96017
Asp208-Leu543
Accession # O96017
Specificity
Detects human Chk2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Chk2 Antibody
Detection of Human Chk2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human Chk2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1358) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Chk2 was detected at approximately 65 kDa (as indicated). For additional reference, duplicate lysates were probed following incubation of the primary antibody with Recombinant Human Chk2 (right panel, Catalog # 1358-KS). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Chk2 by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Chk2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Chk2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1358) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Chk2 at approximately 68 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human Chk2 Antibody
Application
Recommended Usage
Knockout Validated
Chk2 is specifically detected in HeLa human
cervical epithelial carcinoma parental cell line but is not detectable in
Chk2 knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast adenocarcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Chk2
Long Name
Checkpoint Kinase 2
Alternate Names
CDS1, CHEK2, HuCds1, LFS2, PP1425, Rad53
Gene Symbol
CHEK2
UniProt
Additional Chk2 Products
Product Documents for Human Chk2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Chk2 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars