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Human CIN85/SH3KBP1 Antibody

Catalog #: MAB8877 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8877
MAB8877-SP

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Detection of Human CIN85/SH3KBP1 by Western Blot.
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Human CIN85/SH3KBP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CIN85/SH3KBP1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 931308
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CIN85/SH3KBP1
Pro366-Lys665
Accession # Q96B97
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CIN85/SH3KBP1 antibody by Western Blot. View Larger

Detection of Human CIN85/SH3KBP1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CIN85/SH3KBP1 Monoclonal Antibody (Catalog # MAB8877) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CIN85/SH3KBP1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CIN85/SH3KBP1

Independently identified by several groups, SH3-domain kinase-binding protein 1 (SH3KBP1) is also known as CBL-interacting protein of 85 kDa (CIN85), CD2-binding protein 3 (CD2BP3) and human src family kinase-binding protein 1 (HSB1).  SH3KBP1 is a widely expressed adaptor protein involved in regulating diverse signal transduction pathways, including endocytosis, lysosomal degradation, cell adhesion, stress response, cell morphology and cytoskeletal organization.  The protein contains three N-terminal SH3 domains for complex assembly and a C-terminal coiled coil domain for oligomerization, separated by a proline-rich central region.  Smaller known isoforms of SH3KBP1 lack one or more of the SH3 domains.  Over amino acids (aa) 366-665, human SH3KBP1 shares 92% and 90% aa identity with mouse and rat SH3KBP1, respectively.

Long Name
Cbl-Interacting Protein of 85 KDa
Entrez Gene IDs
30011 (Human); 58194 (Mouse); 84357 (Rat)
Alternate Names
C-Cbl-Interacting Protein; CD2-binding protein 3; CD2BP3; CIN85; CIN85Cbl-interacting protein of 85 kDa; GIG10; HSB1; HSB-1; Human Src family kinase-binding protein 1; MIG18; migration-inducing gene 18; SH3 domain-containing kinase-binding protein 1; SH3-domain kinase binding protein 1; SH3KBP1; Src family kinase-binding protein 1; src-related kinase binding protein-1

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