Detection of Claudin‑12 in Caco‑2 Human Cell Line |
Caco‑2 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human Claudin‑12 Monoclonal Antibody (Catalog # MAB4284, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Claudin‑12 in NS0 Mouse Cell Line transfected with human Claudin-12|
Claudin‑12 was detected in immersion fixed NS0 mouse myeloma cell line transfected with human Claudin 12 using 10 µg/mL Mouse Anti-Human Claudin‑12 Monoclonal Antibody (Catalog # MAB4284) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Claudin‑12 is a 244 amino acid multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin‑12 is expressed on intestinal, bladder, and blood‑brain barrier epithelial cells. Human Claudin‑12 shares 92% aa sequence identity with mouse and rat Claudin‑12.