Human CNTF Antibody

Catalog # Availability Size / Price Qty
Human CNTF Antibody in Data
1 Image
Product Details
Citations (4)
Supplemental Products

Human CNTF Antibody Summary

Species Reactivity
Detects human CNTF in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat CNTF is observed and less than 1% cross-reactivity with rhCNTF R alpha is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human CNTF (R&D Systems, Catalog # 257-NT)
Accession # P26441
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human CNTF (Catalog # 257-NT)
Measured by its ability to neutralize CNTF-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND50) is typically 3-8 µg/mL in the presence of 400 ng/mL Recombinant Human CNTF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by CNTF and Neutralization by Human CNTF Antibody. View Larger

Cell Proliferation Induced by CNTF and Neutralization by Human CNTF Antibody. Recombinant Human CNTF (Catalog # 257-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA). The ND50is typically 3-8 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.

The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Long Name
Ciliary Neurotrophic Factor
Entrez Gene IDs
1270 (Human); 12803 (Mouse); 25707 (Rat)
Alternate Names
ciliary neurotrophic factor; CNTF; HCNTF

Product Datasheets

Citations for Human CNTF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Lentiviral-mediated transfer of CNTF to schwann cells within reconstructed peripheral nerve grafts enhances adult retinal ganglion cell survival and axonal regeneration.
    Authors: Leaver SG, Plant GW, Hendriks WT, Niclou SP, Verhaagen J, Harvey AR, Cui Q
    Mol. Ther., 2005;11(6):906-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
    Authors: He W, Gong K, Smith DK, Ip NY
    FEBS Lett., 2005;579(20):4317-23.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
    Authors: He W, Gong K, Zhu G, Smith DK
    FEBS Lett., 2002;514(2):214-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  4. Ciliary neurotrophic factor released by corneal endothelium surviving oxidative stress ex vivo.
    Authors: Koh SW
    Invest. Ophthalmol. Vis. Sci., 2002;43(9):2887-96.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CNTF Antibody

There are currently no reviews for this product. Be the first to review Human CNTF Antibody and earn rewards!

Have you used Human CNTF Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review