Human Common gamma Chain/IL‑2 R gamma Biotinylated Antibody

Name: Human Common gamma Chain/IL‑2 R gamma Biotinylated Antibody
Brand: R&D Systems
SKU: BAF284
Price: 549 USD
Availability: InStock

R&D Systems | Catalog # BAF284

Submit a review

Citations (3)

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry

Cited:

Immunoprecipitation, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Common gamma Chain/IL-2 R gamma Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Common gamma Chain/IL‑2 R gamma
Leu23-Asn254
Accession # P31785

Specificity

Detects Common gamma Chain/IL‑2 R gamma in direct ELISAs and Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) IL-1 sRII, rhIL-2 sR beta, rhIL-3 sR alpha, rhIL-4 sR and rhIL-5 sR alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Common gamma Chain/IL‑2 R gamma Biotinylated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Daudi human Burkitt's lymphoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.1 µg/mL
Sample: Recombinant Human Common gamma Chain/IL‑2 R gamma (Catalog # 384-RG)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma _c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.