Human Common gamma Chain/IL-2 R gamma Antibody
Human Common gamma Chain/IL-2 R gamma Antibody Summary
Leu23-Asn254
Accession # P31785
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by IL‑2 and Neutralization by Human Common gamma Chain/ IL‑2 R gamma Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Common gamma Chain/ IL-2 R gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF284). The ND50 is typically 20-60 µg/mL.
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Detection of Common gamma Chain/IL-2 R gamma by Western Blot Inducible expression of CD127 in monocyte-derived macrophages. (A) Selected immune cells from the innate and adaptive immune system were isolated from Buffy Coats from healthy donors. Some cell types (Neutrophils, CD14+ monocytes, and naive CD4+ T cells were lysed directly after the isolation. Some CD14+ monocytes were matured into Monocyte-derived macrophages, immature and mature DCs as described in the methods section. The amount of CD127 and CD132 was analyzed via Western Blot with beta -Actin as reference. One out of two experiments (n=2) is shown as demonstration. (B) mRNA levels of CD127 and CD132 relative to housekeeping GAPDH mRNA in freshly isolated monocytes and DCs, which were stimulated with 10 ng/ml lipopolysaccharide (LPS) for the displayed time points. Standard deviations of two experiments (n=2) performed with three replicates each are shown. *P < 0.05 for comparison of the indicated time points with baseline. (C) mRNA levels of CD127 and CD132 relative to housekeeping GAPDH mRNA in monocyte-derived macrophages, which were stimulated with 10 ng/ml LPS, 100 ng/ml poly(I:C) HMW or 100 ng/ml pams3CSK4, with or without 5 µg/ml antibody against IFNAR1, for the time points shown. Standard deviations of three experiments (n=3) performed with three replicates each are shown. *P < 0.05 for comparison of the indicated time points with baseline. (D) Monocyte-derived macrophages were stimulated with 10 ng/ml LPS for the displayed time points. The amount of CD127 and CD132 was assessed by Western Blot (top panel) and by immunofluorescence (bottom panel). One out of two experiments (n=2) is shown as demonstration. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33584648), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Common gamma Chain/IL-2 R gamma
The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.
- Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
- Noguchi, M. et al. (1993) Science 262:1877.
Product Datasheets
Citations for Human Common gamma Chain/IL-2 R gamma Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Loss of Interleukin-13-Receptor-Alpha-1 Induces Apoptosis and Promotes EMT in Pancreatic Cancer
Authors: Jingwei Shi, Xiao Shen, Qi Kang, Xing Yang, Maximilian Denzinger, Marko Kornmann et al.
International Journal of Molecular Sciences
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Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
PLoS ONE, 2014-05-12;9(5):e96925.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC-Fr -
Interleukin-7 compartmentalizes its receptor signaling complex to initiate CD4 T lymphocyte response.
Authors: Rose T, Pillet AH, Lavergne V, Tamarit B, Lenormand P, Rousselle JC, Namane A, Theze J
J. Biol. Chem., 2010-02-18;285(20):14898-908.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Immunoprecipitation, Western Blot -
Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
J. Immunol., 2009-06-15;182(12):7389-97.
Species: Human
Sample Types: Plasma
Applications: Immunoprecipitation -
Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
Authors: Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
J. Immunol., 2008-06-01;180(11):7221-9.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity
Authors: Sabrina Rueschenbaum, Chengcong Cai, Matthias Schmidt, Katharina Schwarzkopf, Ulf Dittmer, Stefan Zeuzem et al.
Frontiers in Immunology
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