Human Common gamma Chain/IL-2 R gamma Antibody Summary
Accession # P31785
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑2 and Neutralization by Human Common gamma Chain/ IL‑2 R gamma Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Common ? Chain/ IL-2 R? Antigen Affinity-purified Polyclonal Antibody (Catalog # AF284). The ND50is typically 20-60 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Common gamma Chain/IL-2 R gamma
The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.
- Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
- Noguchi, M. et al. (1993) Science 262:1877.
Citations for Human Common gamma Chain/IL-2 R gamma Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
PLoS ONE, 2014;9(5):e96925.
Sample Types: Whole Cells
Applications: Flow Cytometry
Interleukin-7 compartmentalizes its receptor signaling complex to initiate CD4 T lymphocyte response.
Authors: Rose T, Pillet AH, Lavergne V, Tamarit B, Lenormand P, Rousselle JC, Namane A, Theze J
J. Biol. Chem., 2010;285(20):14898-908.
Sample Types: Cell Lysates
Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
J. Immunol., 2009;182(12):7389-97.
Sample Types: Plasma
Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
Authors: Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
J. Immunol., 2008;180(11):7221-9.
Sample Types: Cell Lysates
Applications: Western Blot
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