Human Common  gamma  Chain/IL‑2 R gamma Antibody

Cell Proliferation Induced by IL‑2 and Neutralization by Human Common gamma Chain/ IL‑2 R gamma Antibody.

Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Common gamma Chain/ IL-2 R gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF284). The ND₅₀ is typically 20-60 µg/mL.

Detection of Common gamma Chain/IL-2 R gamma by Western Blot

Inducible expression of CD127 in monocyte-derived macrophages. (A) Selected immune cells from the innate and adaptive immune system were isolated from Buffy Coats from healthy donors. Some cell types (Neutrophils, CD14+ monocytes, and naive CD4+ T cells were lysed directly after the isolation. Some CD14+ monocytes were matured into Monocyte-derived macrophages, immature and mature DCs as described in the methods section. The amount of CD127 and CD132 was analyzed via Western Blot with beta -Actin as reference. One out of two experiments (n=2) is shown as demonstration. (B) mRNA levels of CD127 and CD132 relative to housekeeping GAPDH mRNA in freshly isolated monocytes and DCs, which were stimulated with 10 ng/ml lipopolysaccharide (LPS) for the displayed time points. Standard deviations of two experiments (n=2) performed with three replicates each are shown. *P < 0.05 for comparison of the indicated time points with baseline. (C) mRNA levels of CD127 and CD132 relative to housekeeping GAPDH mRNA in monocyte-derived macrophages, which were stimulated with 10 ng/ml LPS, 100 ng/ml poly(I:C) HMW or 100 ng/ml pams3CSK4, with or without 5 µg/ml antibody against IFNAR1, for the time points shown. Standard deviations of three experiments (n=3) performed with three replicates each are shown. *P < 0.05 for comparison of the indicated time points with baseline. (D) Monocyte-derived macrophages were stimulated with 10 ng/ml LPS for the displayed time points. The amount of CD127 and CD132 was assessed by Western Blot (top panel) and by immunofluorescence (bottom panel). One out of two experiments (n=2) is shown as demonstration. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33584648), licensed under a CC-BY license. Not internally tested by R&D Systems.