Human Complement Component C1qC Antibody Summary
Accession # P02747
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Complement Component C1qC in Human HUVEC. Complement Component C1qC was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Complement Component C1qC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3337) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C1qC
C1qC is a globular secreted protein and is a subunit of complement component C1q. C1q is composed of 18 polypeptide chains of three distinct subtypes (C1qA, C1qB and C1qC) arranged as 6 disulfide-linked A-B heterodimers and 3 disulfide-linked C-C homodomers. All C1q subunits contain a C1q globular domain and one collagen-like domain. C1q is a subcomponent of complement C1 complex which comprises one C1q and the Ca2+-dependent serine protease tetramer C1r2-C1s2. C1 complex triggers the classical complement pathway. Within the C1q domain, human C1qC shares approximately 75% amino acid sequence identity with mouse and rat C1qC.
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