Human Complement Component C5aR1 Antibody
Human Complement Component C5aR1 Antibody Summary
Accession # P21730
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
N-Acetyl-beta -D-Glucosa-minidase Release Induced by Complement Component C5a and Neutralization by Human Complement Component C5a R1 Antibody. Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N-acetyl-beta -D-glucosa-minidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta -D-Glucosaminidase Release elicited by Recombinant Human Complement Component C5a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Complement Component C5a R1 Monoclonal Antibody (Catalog # MAB3648). The ND50 is typically 2-10 µg/mL in the presence of cytochalasin-B.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C5aR1
Complement Component C5a R1 (C5aR1), also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5aR binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5aR1/C5a interactions are protective during allergen sensitization. Human C5aR1 shares 66% amino acid sequence identity with mouse and rat C5aR.
Citations for Human Complement Component C5aR1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Host-Receptor Post-Translational Modifications Refine Staphylococcal Leukocidin Cytotoxicity
Authors: AT Tromp, M Van Gent, JP Jansen, LM Scheepmake, A Velthuizen, CJC De Haas, KPM Van Kessel, BW Bardoel, M Boettcher, MT McManus, JAG Van Strijp, RJ Lebbink, PA Haas, AN Spaan
Toxins (Basel), 2020-02-06;12(2):.
Sample Types: Whole Cells
Applications: Flow Cytometry
C5a and C5aR are elevated in joints of rheumatoid and psoriatic arthritis patients, and C5aR blockade attenuates leukocyte migration to synovial fluid.
Authors: Hornum L, Hansen A, Tornehave D, Fjording M, Colmenero P, Watjen I, Soe Nielsen N, Bliddal H, Bartels E
PLoS ONE, 2017-12-08;12(12):e0189017.
Sample Types: Whole Tissue
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