Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 347214
View all Complement Component C5aR1 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Complement Component C5a R1
Met1-Val350
Accession # P21730

Specificity

Detects human Complement Component C5a R1. Stains human Complement Component C5a R1 transfected cells but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Complement Component C5aR1 Antibody

N-Acetyl-beta -D-Glucosa-minidase Release Induced by Complement Component C5a and Neutralization by Human Complement Component C5a R1 Antibody.

N-Acetyl-beta -D-Glucosa-minidase Release Induced by Complement Component C5a and Neutralization by Human Complement Component C5a R1 Antibody.

Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N-acetyl-beta -D-glucosa-minidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta -D-Glucosaminidase Release elicited by Recombinant Human Complement Component C5a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Complement Component C5a R1 Monoclonal Antibody (Catalog # MAB3648). The ND50 is typically 2-10 µg/mL in the presence of cytochalasin-B.

Applications for Human Complement Component C5aR1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood granulocytes

Neutralization

Measured by its ability to neutralize Complement Component C5a-induced N-acetyl-beta -D-glucosaminidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Human Complement Component C5a and cytochalasin‑B.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Component C5aR1

Complement Component C5a R1 (C5aR1), also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5aR binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5aR1/C5a interactions are protective during allergen sensitization. Human C5aR1 shares 66% amino acid sequence identity with mouse and rat C5aR.

Long Name

Complement Component C5a Receptor 1

Alternate Names

C5AR1, CD88, Complement Component C5a R1

Entrez Gene IDs

728 (Human); 12273 (Mouse)

Gene Symbol

C5AR1

UniProt

P21730

Additional Complement Component C5aR1 Products

Product Documents for Human Complement Component C5aR1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Complement Component C5aR1 Antibody

For research use only

Citations for Human Complement Component C5aR1 Antibody

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