Human CXCL9/MIG Antibody

Catalog # Availability Size / Price Qty
AF392
AF392-SP
Chemotaxis Induced by CXCL9/MIG and Neutral-ization by Human CXCL9/ MIG Antibody.
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Citations (13)
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Human CXCL9/MIG Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL9/MIG in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse CXCL9 (non-reducing conditions) is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CXCL9/MIG
Thr23-Thr125
Accession # Q07325
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CXCL9/MIG (Catalog # 392-MG)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize CXCL9/MIG-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND50) is typically 5-40 µg/mL in the presence of 1 μg/mL Recombinant Human CXCL9/MIG.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL9/MIG and Neutral-ization by Human CXCL9/ MIG Antibody. View Larger

Chemotaxis Induced by CXCL9/MIG and Neutral-ization by Human CXCL9/ MIG Antibody. Recombinant Human CXCL9/ MIG (392-MG) chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL9/ MIG (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF392). The ND50 is typically 5-40 µg/mL.

Immunocytochemistry CXCL9/MIG antibody in THP-1 Human Cell Line by Immunocytochemistry (ICC). View Larger

CXCL9/MIG in THP‑1 Human Cell Line. CXCL9/MIG was detected in immersion fixed THP-1 human acute monocytic leukemia cell line stimulated with IFN-gamma using Goat Anti-Human CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF392) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Detection of Human CXCL9/MIG by Immunohistochemistry View Larger

Detection of Human CXCL9/MIG by Immunohistochemistry CD8+ T- and CD20+ B-lymphocytes are not localized within inflammatory foci.Immunohistochemistry using anti-CD20 identified a few B-lymphocytes in the inflammatory foci (arrows) (A). In contrast, no CD8+ T-lymphocytes are localized using anti-CD8 antibody (B) (400X). Immunohistrochemistry using anti-pro-IL-18 (C) or anti-CXCL9 (D) reveals the expression of these molecules on mononuclear cells within inflammatory foci (200X). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0014429), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human CXCL9/MIG by Immunohistochemistry View Larger

Detection of Human CXCL9/MIG by Immunohistochemistry CD8+ T- and CD20+ B-lymphocytes are not localized within inflammatory foci.Immunohistochemistry using anti-CD20 identified a few B-lymphocytes in the inflammatory foci (arrows) (A). In contrast, no CD8+ T-lymphocytes are localized using anti-CD8 antibody (B) (400X). Immunohistrochemistry using anti-pro-IL-18 (C) or anti-CXCL9 (D) reveals the expression of these molecules on mononuclear cells within inflammatory foci (200X). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0014429), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL9/MIG

CXCL9, a member of the alpha  subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse MIG cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid residue precursor protein with a 22 amino acid residue signal peptide that is cleaved to yield a 103 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes. The E. coli-expressed CXCL9 preparations produced at R&D Systems have been shown to contain greater than 80% full length CXCL9.

References
  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Liao, F. et al. (1995) J. Exp. Med. 182:1301.
  3. Vanguri, P. (1995) J. Neuroimmunol. 56:35.
Entrez Gene IDs
4283 (Human); 17329 (Mouse); 246759 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma

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Citations for Human CXCL9/MIG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. M1hot tumor-associated macrophages boost tissue-resident memory T cells infiltration and survival in human lung cancer
    Authors: Garrido-Martin EM, Mellows TWP, Clarke J et al.
    J Immunother Cancer
  2. Non-redundant requirement for CXCR3 signalling during tumoricidal T-cell trafficking across tumour vascular checkpoints
    Authors: M. E. Mikucki, D. T. Fisher, J. Matsuzaki, J. J. Skitzki, N. B. Gaulin, J. B. Muhitch et al.
    Nature Communications
  3. Lack of CD8+ T-cell co-localization with Kaposi's sarcoma-associated herpesvirus infected cells in Kaposi's sarcoma tumors
    Authors: SJ Lidenge, FY Tso, O Ngalamika, J Kolape, JR Ngowi, J Mwaiselage, C Wood, JT West
    Oncotarget, 2020-04-28;11(17):1556-1572.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata
    J Immunol, 2016-07-13;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
    Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
    BMC Cancer, 2014-02-22;14(0):118.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Expression and regulation of chemokines in murine and human type 1 diabetes.
    Authors: Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
    Diabetes, 2011-12-30;61(2):436-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Myocarditis in CD8-depleted SIV-infected rhesus macaques after short-term dual therapy with nucleoside and nucleotide reverse transcriptase inhibitors.
    Authors: Annamalai L, Westmoreland SV, Domingues HG
    PLoS ONE, 2010-12-23;5(12):e14429.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Inflammation and tissue repair markers distinguish the nodular sclerosis and mixed cellularity subtypes of classical Hodgkin's lymphoma.
    Authors: Birgersdotter A, Baumforth KR, Porwit A, Sjoberg J, Wei W, Bjorkholm M, Murray PG, Ernberg I
    Br. J. Cancer, 2009-09-22;101(8):1393-401.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
    Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
    J. Immunol., 2009-03-15;182(6):3919-27.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus.
    Authors: Meller S, Winterberg F, Gilliet M, Muller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bunemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B
    Arthritis Rheum., 2005-05-01;52(5):1504-16.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
    Authors: Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M
    Int. J. Oncol., 2004-09-01;25(3):605-13.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
    Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
    J. Immunol., 2002-12-01;169(11):6515-21.
    Species: Human
    Sample Types: BALF, Whole Tissue
    Applications: ELISA Development, IHC-P
  13. Keratinocyte-Derived Chemokines Orchestrate T-Cell Positioning in the Epidermis during Vitiligo and May Serve as Biomarkers of Disease
    Authors: Jillian M. Richmond, Dinesh S. Bangari, Kingsley I. Essien, Sharif D. Currimbhoy, Joanna R. Groom, Amit G. Pandya et al.
    Journal of Investigative Dermatology

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