Human Cystatin C Antibody Summary
Accession # P01034
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Cystatin C by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, and human serum. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cystatin C Monoclonal Antibody (Catalog # MAB1196) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cystatin C at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Cystatin C by Western Blot. Western blot shows lysates of human placenta tissue and PC-3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cystatin C Monoclonal Antibody (Catalog # MAB1196) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cystatin C at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin C
Cystatin C is a member of family 2 of the Cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). It is ubiquitous in human tissues and body fluids. A point mutation in the gene coding for the 120 amino acid mature Cystatin C causes a hereditary form of amyloid angiopathy in which the protein variant (Leu68 to Gln) is deposited in the cerebral arteries, leading to fatal cerebral hemorrhage (4). Cystatin C may have additional clinical applications. For example, it is a good marker for glomerular filtration rate (5).
- Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
- Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Abrahamson, M. et al. (1992) Hum. Genet. 89:377.
- Laterza, O.F. et al. (2002) Clin. Chem. 48:699.
Citations for Human Cystatin C Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Regulation of TGF-beta1-driven differentiation of human lung fibroblasts: emerging roles of cathepsin B and cystatin C.
Authors: Kasabova M, Joulin-Giet A, Lecaille F, Gilmore B, Marchand-Adam S, Saidi A, Lalmanach G
J Biol Chem, 2014;289(23):16239-51.
Sample Types: Cell Lysates
Applications: Western Blot
Dysregulation of macrophage-secreted cathepsin B contributes to HIV-1-linked neuronal apoptosis.
Authors: Rodriguez-Franco EJ, Cantres-Rosario YM, Plaud-Valentin M, Romeu R, Rodriguez Y, Skolasky R, Melendez V, Cadilla CL, Melendez LM
PLoS ONE, 2012;7(5):e36571.
Sample Types: Whole Cells
Cystatin B as a tissue and urinary biomarker of bladder cancer recurrence and disease progression.
Authors: Feldman AS, Banyard J, Wu CL, McDougal WS, Zetter BR
Clin. Cancer Res., 2009;15(3):1024-31.
Sample Types: Urine
Applications: Western Blot
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