Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 201805
View all Cystatin S Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cystatin S
Ser21-Ala141
Accession # P01036

Specificity

Detects human Cystatin S in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Cystatin SA is observed and no cross-reactivity with rhCystatin D, E, SN, rhFetuin A, rhFetuin B, or rhHPRG (Histidine-Proline-Rich Glycoprotein) is observed. In Western blots, approximately 15% cross‑reactivity with rhCystatins SA and SN (under reducing conditions only) is observed and no cross‑reactivity with rhCystatin A, B, C, D, E/M, rhFetuin A, rhFetuin B, or rhHPRG is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Cystatin S Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cystatin S (Catalog # 1296-PI), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Cystatin S (Catalog # 1296-PI)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cystatin S

Cystatin S is a member of family 2 of the Cystatin superfamily (1). Like Cystatins SA and SN, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Unlike Cystatins SA and SN, Cystatin S is not a potent inhibitor of cysteine proteases such as papain and some cathepsins (3). Cystatin S is partially phosphorylated at Ser21 and Ser23 in saliva (4). The functions of Cystatin S are largely unknown. However, it is able to bind more calcium and bind more rapidly to carbonated apatite than cystatins SA or SN, indicating that Cystatin S may be involved in the mineral balance of the tooth (3).

References

  1. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  2. Baron, A.C. et al. (1999) Oral. Dis. 5:344.
  3. Baron, A. et al. (1999) Oral Dis. 5:234.
  4. Isemura, S. et al. (1991) J. Biochem. 110:648.

Alternate Names

CST4

Entrez Gene IDs

1472 (Human)

Gene Symbol

CST4

UniProt

P01036

Additional Cystatin S Products

Product Documents for Human Cystatin S Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Cystatin S Antibody

For research use only

Related Research Areas

Cysteine Proteases and Regulators

Citations for Human Cystatin S Antibody

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