Cytokeratin 19 in MDA‑MB‑231 Human Cell Line.
Cytokeratin 19 was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB3506) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Cytokeratin 19 in Human Breast Cancer Tissue.
Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB3506) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytokeratin 19
Cytokeratin 19, a type I intermediate filament protein, is the smallest
known acidic cytokeratin. The degree of keratin 19 positivity in breast
cancer distinguishes malignant from benign tumours.
Entrez Gene IDs:
3880 (Human); 16669 (Mouse)
CK19; CK-19; Cytokeratin 19; Cytokeratin-19,40-kDa keratin intermediate filament; EndoC; K19; K19cytokeratin-19; K1CS; keratin 19; keratin, type I cytoskeletal 19; keratin, type I, 40-kd; keratin-19; Krt19; MGC15366
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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.